Adenosine A2a Receptor antibody (AA 144-410)

Catalog No. ABIN7870718

This Rabbit Polyclonal antibody specifically detects Adenosine A2a Receptor in WB, ELISA and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for Adenosine A2a Receptor antibody (AA 144-410) (ABIN7870718)

Target: Adenosine A2a Receptor (ADORA2A)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Adenosine A2a Receptor antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-Adenosine A2a Receptor Antibody

Binding Specificity: AA 144-410

Purpose: ADORA2A Antibody / Adenosine receptor A2A

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human ADORA2A recombinant protein (Position: N144-G410) was used as the immunogen for the ADORA2A antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the ADORA2A antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the ADORA2A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Adenosine A2a Receptor

Target: Adenosine A2a Receptor (ADORA2A)

Alternative Name: ADORA2A

Background: ADORA2A antibody recognizes Adenosine receptor A2A, a G protein-coupled receptor (GPCR) that mediates adenosine signaling to regulate neurotransmission, vascular tone, and immune responses. The ADORA2A gene, located on chromosome 22q11.23, encodes a seven-transmembrane domain receptor coupled to Gs proteins that stimulate cyclic AMP (cAMP) production upon adenosine binding. This receptor is highly expressed in the central nervous system, particularly in the striatum, as well as in immune cells, endothelial cells, and platelets.

Structurally, ADORA2A consists of an extracellular ligand-binding pocket for adenosine and intracellular domains that interact with G proteins and regulatory kinases. The receptor plays a critical role in modulating dopaminergic neurotransmission, motor control, and sleep regulation. Pharmacologically, ADORA2A is a therapeutic target for Parkinson's disease and psychiatric disorders due to its antagonistic interaction with dopamine D2 receptors. In the cardiovascular system, ADORA2A activation causes vasodilation and platelet inhibition, contributing to tissue perfusion and anti-thrombotic effects.

ADORA2A signaling also exerts immunomodulatory functions by suppressing pro-inflammatory cytokine production and promoting regulatory T cell activity. Dysregulation of this receptor is linked to neurodegenerative diseases, ischemia, and autoimmune conditions. Overexpression of ADORA2A in certain tumors supports immunosuppressive microenvironments by inhibiting T-cell activation, making it an emerging target for cancer immunotherapy. Structural and mutational analyses have revealed key residues responsible for ligand specificity and receptor desensitization through phosphorylation and arrestin binding.

Immunohistochemical staining using ADORA2A antibody shows membrane and cytoplasmic localization in neurons, endothelial cells, and lymphocytes. ADORA2A antibody from NSJ Bioreagents provides a powerful tool for studying adenosine-mediated signaling, neuroprotection, cardiovascular regulation, and tumor immunology.

UniProt: P29274

Pathways: Neurotrophin Signaling Pathway, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints