Transglutaminase 5 antibody (AA 146-608)

Catalog No. ABIN7870734

The Rabbit Polyclonal anti-Transglutaminase 5 antibody is suitable to detect Transglutaminase 5 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and FACS.

Quick Overview for Transglutaminase 5 antibody (AA 146-608) (ABIN7870734)

Target: Transglutaminase 5 (TGM5)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Transglutaminase 5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-Transglutaminase 5 Antibody

Binding Specificity: AA 146-608

Purpose: TGM5 Antibody / Transglutaminase 5

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human TGM5 recombinant protein (Position: E146-K608) was used as the immunogen for the TGM5 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TGM5 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the TGM5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Transglutaminase 5

Target: Transglutaminase 5 (TGM5)

Alternative Name: TGM5

Background: TGM5 antibody detects Transglutaminase-5, encoded by the TGM5 gene on chromosome 15q15.2. TGM5 antibody is used in research on epidermal biology, keratinocyte differentiation, and skin barrier formation. TGM5 belongs to the transglutaminase family of Ca2+-dependent enzymes that catalyze crosslinking of proteins via isopeptide bonds. TGM5 is expressed primarily in epidermis and other stratified epithelia, where it contributes to cornified envelope formation, strengthening skin barrier integrity. Mutations in TGM5 cause acral peeling skin syndrome, highlighting its essential role.

Structurally, TGM5 is a ~78 kDa enzyme with an N-terminal beta-sandwich domain, a catalytic core containing the conserved transglutaminase triad, and two C-terminal beta-barrel domains. The catalytic cysteine forms thioester intermediates during transamidation reactions. Structural similarities to other transglutaminases confirm conserved mechanisms but tissue-specific regulation.

Functionally, TGM5 catalyzes crosslinking of structural proteins including involucrin, loricrin, and small proline-rich proteins. This activity stabilizes the cornified envelope of keratinocytes, ensuring epidermal resilience and barrier function. Knockdown or mutation disrupts barrier integrity, leading to peeling and sensitivity. Researchers employ TGM5 antibody to study keratinocyte biology, epidermal differentiation, and barrier formation.

Clinically, TGM5 mutations cause acral peeling skin syndrome, characterized by painless shedding of the outer epidermis. Pathogenic variants impair catalytic activity, disrupting keratinocyte crosslinking. TGM5 is also studied in cancer, where altered expression influences differentiation and tumor progression. It may serve as a biomarker of keratinocyte differentiation status in skin diseases. NSJ Bioreagents supplies TGM5 antibody as a reagent for dermatology and barrier research.

Experimentally, TGM5 antibody is applied in western blotting to detect the ~78 kDa protein, in immunohistochemistry to study epidermal localization, and in immunofluorescence to visualize keratinocyte differentiation. Enzyme activity assays combined with TGM5 antibody confirm correlations between expression and functional crosslinking activity.

UniProt: O43548