DAK antibody (AA 15-538)

Catalog No. ABIN7870797

The Rabbit Polyclonal anti-DAK antibody is suitable to detect DAK in samples from Human, Mouse and Rat. It has been validated for ELISA, WB, IHC, IF and FACS.

Quick Overview for DAK antibody (AA 15-538) (ABIN7870797)

Target: DAK (Dihydroxyacetone Kinase 2 Homolog (DAK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAK antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-DAK Antibody

Binding Specificity: AA 15-538

Purpose: TKFC Antibody / Triokinase/FMN cyclase

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human DAK/TKFC recombinant protein (Position: D15-E538) was used as the immunogen for the TKFC antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TKFC antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the TKFC antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for DAK

Target: DAK (Dihydroxyacetone Kinase 2 Homolog (DAK))

Alternative Name: TKFC

Background: TKFC antibody detects Triokinase/FMN cyclase, a bifunctional enzyme involved in carbohydrate and flavin metabolism. TKFC catalyzes phosphorylation of glyceraldehyde and dihydroxyacetone to glyceraldehyde 3-phosphate and forms cyclic FMN from FMN, linking sugar and cofactor metabolism. The TKFC antibody is widely used in metabolic and enzymology research to study carbohydrate utilization, vitamin metabolism, and redox regulation.

TKFC is encoded by the TKFC gene located on human chromosome 17p13.1. The protein is approximately 585 amino acids long and localizes to the cytoplasm, particularly in liver and kidney tissues, where it supports energy metabolism and flavin cofactor recycling. TKFC functions as a bifunctional enzyme with separate kinase and cyclase domains, ensuring efficient coordination between sugar phosphorylation and FMN homeostasis.

The TKFC antibody detects a 63 kilodalton protein by western blot and shows cytoplasmic staining under immunofluorescence microscopy. TKFC participates in glycerol metabolism by converting trioses into intermediates of glycolysis and gluconeogenesis, thereby contributing to glucose homeostasis. Its FMN cyclase activity recycles flavin cofactors that support oxidative enzymes and mitochondrial redox balance.

Defects in TKFC activity lead to impaired energy metabolism, elevated triose intermediates, and disturbances in flavin homeostasis. Although rare, TKFC deficiency has been linked to metabolic acidosis and hepatic dysfunction. Elevated TKFC expression in tumors reflects metabolic adaptation to high glycolytic flux and enhanced redox cycling, supporting cell proliferation under hypoxia.

By connecting carbohydrate utilization and flavin metabolism, TKFC provides a unique window into cellular bioenergetics and redox control. NSJ Bioreagents provides a validated TKFC antibody optimized for western blot, immunofluorescence, and metabolic profiling, supporting research into energy metabolism, flavin biochemistry, and metabolic regulation.

UniProt: Q3LXA3