TBR1 antibody (AA 15-682)

Catalog No. ABIN7870804

The Rabbit Polyclonal anti-TBR1 antibody (ABIN7870804) specifically detects TBR1 in WB, ELISA, IHC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for TBR1 antibody (AA 15-682) (ABIN7870804)

Target: TBR1 (T-Box Brain Gene 1 (TBR1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TBR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-TBR1 Antibody

Binding Specificity: AA 15-682

Purpose: TBR1 Antibody / T-box brain protein 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human TBR1 recombinant protein (Position: K15-S682) was used as the immunogen for the TBR1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TBR1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the TBR1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for TBR1

Target: TBR1 (T-Box Brain Gene 1 (TBR1))

Alternative Name: TBR1

Background: TBR1 antibody detects T-box brain protein 1, a neuron-specific transcription factor that regulates cortical development and neuronal differentiation. The UniProt recommended name is T-box brain protein 1 (TBR1). This DNA-binding transcription factor belongs to the T-box family and is essential for neocortical layer specification, axonal guidance, and synaptic connectivity.

Functionally, TBR1 antibody identifies a 682-amino-acid nuclear protein that binds T-box DNA consensus sequences to regulate the expression of genes involved in neuronal subtype identity and projection targeting. TBR1 interacts with transcriptional co-regulators such as CASK, FOXP2, and SOX5, forming complexes that guide early cortical neuron differentiation and maturation.

The TBR1 gene is located on chromosome 2q24.2 and is expressed predominantly in postmitotic glutamatergic neurons of the developing and adult cerebral cortex. It functions as a key determinant of cortical layer VI identity and regulates axonal projections to subcortical targets. TBR1 is a downstream effector of the transcription factor PAX6, linking progenitor differentiation to neuronal specialization.

Pathologically, TBR1 mutations have been associated with autism spectrum disorders, intellectual disability, and cortical malformations. Its dysregulation disrupts neuronal migration, synapse formation, and interhemispheric connectivity. Research using TBR1 antibody provides insights into neurodevelopmental biology, transcriptional regulation, and disease mechanisms involving cortical circuitry.

TBR1 antibody is suitable for western blotting, immunofluorescence, and immunohistochemistry to label developing cortical neurons and nuclear transcription factors. NSJ Bioreagents offers validated TBR1 antibody reagents optimized for studies in neurogenesis, brain development, and transcriptional control.

Structurally, T-box brain protein 1 contains a central T-box DNA-binding domain that mediates sequence-specific gene regulation. It acts as both an activator and repressor depending on its interacting partners. This antibody enables researchers to explore TBR1's roles in cortical specification and neural differentiation.

UniProt: Q16650