USP15 antibody (AA 151-180)

Catalog No. ABIN7870973

This Rabbit Polyclonal antibody specifically detects USP15 in WB, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for USP15 antibody (AA 151-180) (ABIN7870973)

Target: USP15 (Ubiquitin Specific Peptidase 15 (USP15))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP15 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-USP15 Antibody

Binding Specificity: AA 151-180

Purpose: USP15 Antibody

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 151-180 from the human protein was used as the immunogen for the USP15 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the USP15 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the USP15 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for USP15

Target: USP15 (Ubiquitin Specific Peptidase 15 (USP15))

Alternative Name: USP15

Background: Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

UniProt: Q9Y4E8