MINPP1 antibody (AA 155-421)

Catalog No. ABIN7871010

The Rabbit Polyclonal anti-MINPP1 antibody is suitable to detect MINPP1 in samples from Human, Rat and Mouse. It has been validated for ELISA, WB, IHC and FACS.

Quick Overview for MINPP1 antibody (AA 155-421) (ABIN7871010)

Target: MINPP1 (Multiple Inositol-Polyphosphate Phosphatase 1 (MINPP1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MINPP1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-MINPP1 Antibody

Binding Specificity: AA 155-421

Purpose: MINPP1 Antibody / Multiple inositol polyphosphate phosphatase 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human MINPP1 recombinant protein (Position: R155-H421) was used as the immunogen for the MINPP1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MINPP1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MINPP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MINPP1

Target: MINPP1 (Multiple Inositol-Polyphosphate Phosphatase 1 (MINPP1))

Alternative Name: MINPP1

Background: MINPP1 antibody detects Multiple inositol polyphosphate phosphatase 1, encoded by the MINPP1 gene on chromosome 10q23.31. MINPP1 antibody is used in studies of inositol phosphate metabolism, signaling, and cancer biology. MINPP1 is a phosphatase localized to the endoplasmic reticulum (ER) lumen that dephosphorylates higher-order inositol polyphosphates, including IP4, IP5, and IP6, generating lower-phosphate species. This activity regulates cell signaling pathways controlling proliferation, apoptosis, and stress responses.

Structurally, MINPP1 is a ~56 kDa protein with a phosphatase domain belonging to the histidine phosphatase superfamily. It contains a signal peptide for ER targeting and a catalytic domain with conserved histidine residues required for enzymatic activity. Isoforms generated by alternative splicing show distinct expression patterns in different tissues.

Functionally, MINPP1 regulates intracellular signaling by hydrolyzing inositol polyphosphates that act as secondary messengers. It influences calcium release, apoptosis, and metabolic signaling. In the ER, MINPP1 modulates cellular sensitivity to stress and nutrient availability. Knockout or loss of activity alters polyphosphate metabolism, impairing growth and survival. Researchers use MINPP1 antibody to study phosphoinositide metabolism, ER stress responses, and cancer pathways.

Clinically, MINPP1 mutations cause pontocerebellar hypoplasia type 14, a neurodevelopmental disorder with intellectual disability, seizures, and motor deficits. Altered expression has been linked to glioblastoma and prostate cancer, where MINPP1 influences proliferation and survival. As phosphoinositide signaling is a therapeutic target, MINPP1 is of interest in oncology and neurology. NSJ Bioreagents offers MINPP1 antibody to support research in metabolism, neurodevelopment, and cancer biology.

Experimentally, MINPP1 antibody is used in western blotting to detect the ~56 kDa protein, in immunohistochemistry to examine brain and tumor tissue, and in immunofluorescence microscopy to visualize ER localization. Enzyme activity assays combined with MINPP1 antibody allow correlation of expression with phosphatase activity.

UniProt: Q9UNW1