SETD7 antibody (AA 159-189)

Catalog No. ABIN7871034

The Rabbit Polyclonal anti-SETD7 antibody is suitable to detect SETD7 in samples from Human and Mouse. It has been validated for WB and IHC (p).

Quick Overview for SETD7 antibody (AA 159-189) (ABIN7871034)

Target: SETD7 (SET Domain Containing (Lysine Methyltransferase) 7 (SETD7))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SETD7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SETD7 Antibody

Binding Specificity: AA 159-189

Purpose: SETD7 Antibody / SET7

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 159-189 from the human protein was used as the immunogen for the SETD7 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the SETD7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the SETD7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for SETD7

Target: SETD7 (SET Domain Containing (Lysine Methyltransferase) 7 (SETD7))

Alternative Name: SETD7

Background: Similar to acetylation and phosphorylation, histone methylation at the N-terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family. The retinoblastoma protein-interacting zinc finger geneRIZ1 is a tumor suppressor gene and a FOUNDING member of the PR domain family. RIZ1 inactivation is commonly found in many types of human cancers and occurs through loss of mRNA expression, frame shift mutation, chromosomal deletion, and missense mutation. RIZ1 is also a tumor susceptibility gene in mice. The loss of RIZ1 mRNA in human cancers was shown to associate with DNA methylation of its promoter CpG island. Methylation of the RIZ1 promoter strongly correlated with lost or decreased RIZ1 mRNA expression in breast, liver, colon, and lung cancer cell lines as well as in liver cancer tissues.

UniProt: Q8WTS6