HAPLN1 antibody (AA 16-354)

Catalog No. ABIN7871070

The Rabbit Polyclonal anti-HAPLN1 antibody is suitable to detect HAPLN1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC and ELISA.

Quick Overview for HAPLN1 antibody (AA 16-354) (ABIN7871070)

Target: HAPLN1 (Hyaluronan and Proteoglycan Link Protein 1 (HAPLN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HAPLN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-HAPLN1 Antibody

Binding Specificity: AA 16-354

Purpose: HAPLN1 Antibody / Hyaluronan and proteoglycan link protein 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human HAPLN1 recombinant protein (Position: D16-N354) was used as the immunogen for the HAPLN1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the HAPLN1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the HAPLN1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for HAPLN1

Target: HAPLN1 (Hyaluronan and Proteoglycan Link Protein 1 (HAPLN1))

Alternative Name: HAPLN1

Background: HAPLN1 antibody detects Hyaluronan and proteoglycan link protein 1, an extracellular matrix (ECM) glycoprotein essential for stabilizing aggregates of hyaluronan and chondroitin sulfate proteoglycans. HAPLN1 promotes structural integrity of cartilage, brain ECM, and connective tissue by maintaining the association between hyaluronan and aggrecan. The HAPLN1 antibody is widely used in developmental biology, neurobiology, and cartilage research to examine ECM organization and tissue morphogenesis.

HAPLN1 is encoded by the HAPLN1 gene on human chromosome 5q14.3. The protein is approximately 38 kilodaltons and belongs to the link protein family characterized by tandem immunoglobulin-like domains. HAPLN1 forms ternary complexes with hyaluronan and aggrecan, contributing to the viscoelastic properties of cartilage and the structural framework of the brain extracellular matrix. It is critical for the assembly of perineuronal nets, specialized ECM structures that regulate neuronal excitability and plasticity.

Using the HAPLN1 antibody, researchers can detect the protein by western blot, where it appears as a 38i 1/240 kilodalton band, or by immunohistochemistry, where it localizes to extracellular matrix regions of cartilage, brain, and developing tissues. HAPLN1 expression begins early in embryogenesis, marking sites of cartilage condensation and neural patterning. In adults, it contributes to the stability of mature ECM networks, particularly within articular cartilage and neuronal environments. Loss or alteration of HAPLN1 disrupts ECM cohesion, leading to joint instability or impaired neuronal function.

HAPLN1 has clinical relevance in osteoarthritis, rheumatoid arthritis, and neurodegenerative diseases. Decreased HAPLN1 levels correlate with cartilage degradation and ECM disorganization. In the nervous system, reduced expression is linked to increased synaptic plasticity and neuroinflammation. Conversely, HAPLN1 overexpression may promote fibrotic ECM accumulation. NSJ Bioreagents provides a validated HAPLN1 antibody, supporting research into tissue architecture, ECM remodeling, and matrix-protein interactions in both health and disease.

UniProt: P10915