Dnmt2 antibody (AA 16-391)

Catalog No. ABIN7871074

The Rabbit Polyclonal anti-Dnmt2 antibody (ABIN7871074) specifically detects Dnmt2 in ELISA, WB and FACS. The antibody is reactive with Mouse and Rat samples.

Quick Overview for Dnmt2 antibody (AA 16-391) (ABIN7871074)

Target: Dnmt2 (TRDMT1) (tRNA Aspartic Acid Methyltransferase 1 (TRDMT1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dnmt2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-Dnmt2 Antibody

Binding Specificity: AA 16-391

Purpose: Dnmt2 Antibody / DNA methyltransferase 2 / TRDMT1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived mouse DNMT2/TRDMT1 recombinant protein (Position: M16-E391) was used as the immunogen for the Dnmt2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Dnmt2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Dnmt2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Dnmt2

Target: Dnmt2 (TRDMT1) (tRNA Aspartic Acid Methyltransferase 1 (TRDMT1))

Alternative Name: Dnmt2

Background: DNMT2 antibody detects DNA methyltransferase homolog 2, encoded by the TRDMT1 gene on chromosome 10p15.1. DNMT2 antibody is applied in research on epigenetics, RNA modification, and gene regulation. Although named DNA methyltransferase 2, DNMT2 primarily functions as an RNA methyltransferase that catalyzes cytosine-5 methylation at tRNA-Asp and other tRNAs, influencing translation fidelity and stability. Expression is detected in many tissues, including liver, brain, and hematopoietic cells, and is conserved across eukaryotes, reflecting its evolutionary importance.

Structurally, DNMT2 resembles other DNA methyltransferases, with a catalytic domain containing conserved motifs for S-adenosylmethionine (SAM) binding and cytosine methylation. However, it lacks extended regulatory domains found in DNMT1 and DNMT3, resulting in unique substrate specificity. The protein is ~39 kDa and localized predominantly in the nucleus but also found in cytoplasmic compartments associated with tRNA modification.

Functionally, DNMT2 catalyzes methylation of cytosine residues in tRNA, enhancing their stability and protecting against stress-induced cleavage. By modifying tRNAs, DNMT2 indirectly regulates protein synthesis and cellular stress responses. It has roles in development, hematopoiesis, and innate immunity. Knockout models reveal its importance in protecting against viral infections and maintaining genome stability under stress. Researchers use DNMT2 antibody to investigate RNA modifications, epigenetics, and translational control.

Clinically, DNMT2 has been linked to cancer, neurological disease, and viral infection. Altered expression contributes to tumorigenesis, with context-dependent roles as either tumor suppressor or oncogene. DNMT2 polymorphisms are studied for associations with neurodegenerative disorders and metabolic syndromes. Its antiviral roles suggest relevance for infection biology, as methylated tRNAs resist viral endonuclease degradation. NSJ Bioreagents supplies DNMT2 antibody to support research in epigenetics, RNA biology, and disease mechanisms.

Experimentally, DNMT2 antibody is used in western blotting to detect the ~39 kDa protein, in immunofluorescence to study nuclear and cytoplasmic localization, and in immunohistochemistry to analyze tissue-specific expression. RNA immunoprecipitation with DNMT2 antibody helps identify methylated RNA targets.

UniProt: O55055