TDO2 antibody (AA 16-406)

Catalog No. ABIN7871075

This Rabbit Polyclonal antibody specifically detects TDO2 in WB, IHC, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TDO2 antibody (AA 16-406) (ABIN7871075)

Target: TDO2 (Tryptophan 2,3-Dioxygenase (TDO2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TDO2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-TDO2 Antibody

Binding Specificity: AA 16-406

Purpose: TDO2 Antibody / Tryptophan 2,3-dioxygenase

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human TDO2 recombinant protein (Position: K16-D406) was used as the immunogen for the TDO2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TDO2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the TDO2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for TDO2

Target: TDO2 (Tryptophan 2,3-Dioxygenase (TDO2))

Alternative Name: TDO2

Background: TDO2 antibody detects tryptophan 2,3-dioxygenase, a heme-dependent enzyme that catalyzes the first step of the kynurenine pathway, converting L-tryptophan to N-formylkynurenine. The UniProt recommended name is Tryptophan 2,3-dioxygenase (TDO2). This enzyme regulates systemic tryptophan levels and influences serotonin biosynthesis, immune tolerance, and NAD+ metabolism.

Functionally, TDO2 antibody identifies a homotetrameric enzyme that incorporates molecular oxygen into the indole ring of tryptophan. The enzyme is predominantly expressed in liver but is inducible in many tumor types. TDO2 activity shapes the immune microenvironment by depleting tryptophan and generating immunosuppressive kynurenine metabolites. This mechanism contributes to tumor immune evasion and cancer progression.

The TDO2 gene is located on chromosome 4q32.1 and encodes a 406-amino-acid protein with a conserved heme-binding pocket. Its expression is transcriptionally induced by glucocorticoids, stress, and pro-inflammatory cytokines. Elevated TDO2 levels have been reported in hepatocellular carcinoma, glioblastoma, and melanoma, positioning it as a therapeutic target in oncology. TDO2 also regulates neuroactive tryptophan metabolites implicated in mood disorders and neurodegeneration.

Pathologically, upregulation of TDO2 disturbs immune cell proliferation and antigen presentation through depletion of tryptophan and accumulation of kynurenine. Inhibiting TDO2 restores T-cell activity and is an emerging immunotherapeutic strategy complementary to checkpoint blockade. In metabolic studies, altered TDO2 activity links to hepatic inflammation and altered redox homeostasis.

TDO2 antibody is widely applied in cancer immunology, metabolism, and neurobiology research. It is suitable for immunoblotting, immunohistochemistry, and enzyme assays detecting TDO2 expression or localization. NSJ Bioreagents supplies high-quality TDO2 antibody reagents validated for use in relevant metabolic and immunological assays.

Structurally, tryptophan 2,3-dioxygenase possesses a catalytic heme group coordinated by a proximal histidine. Substrate binding induces conformational changes that enhance dioxygen activation. This antibody supports biochemical and translational studies targeting TDO2 for metabolic modulation and immunotherapy development.

UniProt: P48775