Splicing Factor 1 antibody (AA 160-266)

Catalog No. ABIN7871098

The Mouse Monoclonal anti-Splicing Factor 1 antibody (Clone 2F5D10) (ABIN7871098) specifically detects Splicing Factor 1 in WB, IF, IHC (p) and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Splicing Factor 1 antibody (AA 160-266) (ABIN7871098)

Target: Splicing Factor 1 (SF1)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Splicing Factor 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: 2F5D10

Product Details anti-Splicing Factor 1 Antibody

Binding Specificity: AA 160-266

Purpose: SF1 Antibody / Splicing factor 1

Purification: Affinity purified

Immunogen: Recombinant human protein (amino acids R160-Q266) was used as the immunogen for the SF1 antibody.

Isotype: IgG2a

Application Details

Application Notes: Optimal dilution of the SF1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Splicing Factor 1

Target: Splicing Factor 1 (SF1)

Alternative Name: SF1

Background: Splicing factor 1 also known as zinc finger protein 162 (ZFM162) is a protein that in humans is encoded by the SF1 gene. This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.

UniProt: Q15637

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location