Splicing Factor 1 antibody (AA 160-266)

Catalog No. ABIN7871099

This Mouse Monoclonal antibody specifically detects Splicing Factor 1 in WB, IF, IHC (p) and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Splicing Factor 1 antibody (AA 160-266) (ABIN7871099)

Target: Splicing Factor 1 (SF1)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Splicing Factor 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: 7D9E3

Product Details anti-Splicing Factor 1 Antibody

Binding Specificity: AA 160-266

Purpose: Splicing factor 1 Antibody / SF1

Purification: Affinity purified

Immunogen: Recombinant human protein (amino acids R160-Q266) was used as the immunogen for the Splicing factor 1 antibody.

Isotype: IgG2b

Application Details

Application Notes: Optimal dilution of the Splicing factor 1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Splicing factor 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Splicing Factor 1

Target: Splicing Factor 1 (SF1)

Alternative Name: Splicing factor 1

Background: Splicing factor 1 also known as zinc finger protein 162 (ZFM162) is a protein that in humans is encoded by the SF1 gene. This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.

UniProt: Q15637

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location