CDC45 antibody (AA 166-431)

Catalog No. ABIN7871157

The Mouse Monoclonal anti-CDC45 antibody is suitable to detect CDC45 in samples from Human, Mouse and Rat. It has been validated for WB, FACS and IHC (p).

Quick Overview for CDC45 antibody (AA 166-431) (ABIN7871157)

Target: CDC45 (Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDC45 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 6H6

Product Details anti-CDC45 Antibody

Binding Specificity: AA 166-431

Purpose: PORC-PI-1 Antibody / CDC45

Purification: Affinity purified

Immunogen: A human recombinant partial protein (amino acids E166-A431) was used as the immunogen for the PORC-PI-1 antibody.

Isotype: IgG1

Application Details

Application Notes: Optimal dilution of the PORC-PI-1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PORC-PI-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for CDC45

Target: CDC45 (Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45))

Alternative Name: PORC-PI-1

Background: CDC45 is a protein that in humans is encoded by the CDC45 gene. The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants.

UniProt: O75419

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA