Prohibitin antibody (AA 167-261)

Catalog No. ABIN7871164

The Mouse Monoclonal anti-Prohibitin antibody (Clone PHB-3231) (ABIN7871164) specifically detects Prohibitin in WB, IF and IHC (p). The antibody is reactive with Human samples.

Quick Overview for Prohibitin antibody (AA 167-261) (ABIN7871164)

Target: Prohibitin (PHB)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Prohibitin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: PHB-3231

Product Details anti-Prohibitin Antibody

Binding Specificity: AA 167-261

Purpose: Prohibitin Antibody / PHB (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogen: A recombinant human partial protein corresponding to amino acids 167-261 was used as the immunogen for the Prohibitin antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the Prohibitin antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Prohibitin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Prohibitin

Target: Prohibitin (PHB)

Alternative Name: Prohibitin

Background: This antibody recognizes a protein of 30 kDa which is identified as Prohibitin (PHB), an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although PHB mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. It is an ideal mitochondrial marker. It shows anti-proliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.

UniProt: P35232

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling