MASP1 antibody (AA 169-580)

Catalog No. ABIN7871206

The Rabbit Polyclonal anti-MASP1 antibody (ABIN7871206) specifically detects MASP1 in WB, ELISA and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MASP1 antibody (AA 169-580) (ABIN7871206)

Target: MASP1 (Mannan-Binding Lectin Serine Peptidase 1 (MASP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MASP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-MASP1 Antibody

Binding Specificity: AA 169-580

Purpose: MASP1 Antibody / Mannan-binding lectin serine protease 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human MASP1 recombinant protein (Position: R169-E580) was used as the immunogen for the MASP1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MASP1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MASP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MASP1

Target: MASP1 (Mannan-Binding Lectin Serine Peptidase 1 (MASP1))

Alternative Name: MASP1

Background: MASP1 antibody detects Mannan-binding lectin serine protease 1, encoded by the MASP1 gene on chromosome 3q27.3. MASP1 antibody is commonly used in research on innate immunity, complement activation, and infection biology. MASP1 is a serine protease that associates with mannan-binding lectin (MBL) and ficolins in the lectin pathway of the complement system. It initiates proteolytic cascades leading to opsonization, inflammation, and pathogen clearance. MASP1 is expressed in the liver and circulates in plasma as part of MBL-associated complexes.

Structurally, MASP1 is a ~110 kDa protein composed of CUB domains, EGF-like domains, complement control protein (CCP) repeats, and a C-terminal serine protease domain. Alternative splicing produces multiple isoforms, including MASP1 and MASP3, with distinct activities. These isoforms diversify complement activation roles and are essential for fine-tuning immune responses.

Functionally, MASP1 cleaves complement components C4 and C2, generating C3 convertase in the lectin pathway. It also activates coagulation factors, linking complement with clotting. MASP1 participates in host defense by enhancing pathogen recognition and clearance. Beyond immunity, MASP1 influences angiogenesis and tissue repair. Researchers use MASP1 antibody to study complement activation, innate defense, and coagulation biology.

Clinically, MASP1 variants are associated with susceptibility to infections and inflammatory diseases. Deficiency impairs lectin pathway activation, increasing risk of bacterial and viral infections. Conversely, overactivation of MASP1 contributes to autoimmune diseases, sepsis, and ischemia-reperfusion injury. Mutations in MASP1 are linked to 3MC syndrome, a developmental disorder with craniofacial anomalies and growth defects. As complement therapeutics are developed, MASP1 is an emerging target for intervention. NSJ Bioreagents provides MASP1 antibody for complement biology, infectious disease, and immunology research.

Experimentally, MASP1 antibody is applied in western blotting to detect the ~110 kDa protein, in ELISA to measure plasma levels, and in immunohistochemistry to examine tissue expression. Co-immunoprecipitation with MASP1 antibody identifies binding partners within MBL-associated complexes.

UniProt: P48740

Pathways: Complement System