PIPOX antibody (AA 173-390)

Catalog No. ABIN7871271

The Rabbit Polyclonal anti-PIPOX antibody is suitable to detect PIPOX in samples from Human, Mouse and Rat. It has been validated for ELISA, WB and IHC.

Quick Overview for PIPOX antibody (AA 173-390) (ABIN7871271)

Target: PIPOX (Pipecolic Acid Oxidase (PIPOX))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIPOX antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PIPOX Antibody

Binding Specificity: AA 173-390

Purpose: PIPOX Antibody / Peroxisomal sarcosine oxidase

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human PIPOX recombinant protein (Position: E173-L390) was used as the immunogen for the PIPOX antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PIPOX antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PIPOX antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PIPOX

Target: PIPOX (Pipecolic Acid Oxidase (PIPOX))

Alternative Name: PIPOX

Background: PIPOX antibody detects Peroxisomal sarcosine oxidase, a flavin-dependent enzyme involved in the oxidative demethylation of sarcosine and pipecolate in peroxisomes. The UniProt recommended name is Peroxisomal sarcosine oxidase (PIPOX). This enzyme participates in amino acid catabolism and contributes to cellular redox homeostasis through hydrogen peroxide production during substrate oxidation.

Functionally, PIPOX antibody identifies a 438-amino-acid peroxisomal matrix enzyme that catalyzes the conversion of sarcosine to glycine, formaldehyde, and hydrogen peroxide. PIPOX contains a covalently bound FAD cofactor that mediates electron transfer to molecular oxygen during oxidative demethylation. It also oxidizes L-pipecolate, a lysine degradation intermediate, linking it to amino acid metabolism and detoxification of secondary amines.

The PIPOX gene is located on chromosome 17p13.3 and encodes a protein expressed in liver, kidney, and muscle tissues, where peroxisomal metabolism is active. PIPOX contributes to maintaining amino acid balance and redox stability by coupling substrate oxidation with reactive oxygen species generation. In peroxisomes, PIPOX works alongside catalase to neutralize hydrogen peroxide, preventing oxidative stress accumulation.

Physiologically, PIPOX supports one-carbon metabolism by producing glycine, a precursor for glutathione synthesis and methylation reactions. Its regulation is influenced by dietary amino acid intake, metabolic state, and oxidative stress levels. Reduced PIPOX activity disrupts amino acid degradation and increases susceptibility to oxidative injury. Elevated activity has been observed in metabolic adaptations to fasting and in response to peroxisome proliferator-activated receptor (PPAR) signaling.

PIPOX antibody is widely used in peroxisomal biology, amino acid metabolism, and redox regulation research. It is suitable for western blotting, immunohistochemistry, and enzyme localization assays to study PIPOX expression and function. This antibody supports studies of peroxisomal oxidative pathways, redox signaling, and metabolic adaptation. In biomedical research, PIPOX serves as a marker of peroxisomal enzymatic activity and amino acid turnover.

Structurally, PIPOX contains FAD-binding and substrate-binding domains typical of the flavin oxidase family. It localizes to peroxisomes via a C-terminal peroxisomal targeting signal (PTS1). NSJ Bioreagents provides PIPOX antibody reagents validated for use in amino acid metabolism, oxidative stress, and peroxisomal function research.

UniProt: Q9P0Z9