SOS1 antibody (AA 177-1288)

Catalog No. ABIN7871341

The Rabbit Polyclonal anti-SOS1 antibody (ABIN7871341) specifically detects SOS1 in WB, ELISA, IHC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SOS1 antibody (AA 177-1288) (ABIN7871341)

Target: SOS1 (Son of Sevenless Homolog 1 (SOS1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-SOS1 Antibody

Binding Specificity: AA 177-1288

Purpose: SOS1 Antibody / Son of sevenless 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SOS1 recombinant protein (Position: H177-A1288) was used as the immunogen for the SOS1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SOS1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SOS1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SOS1

Target: SOS1 (Son of Sevenless Homolog 1 (SOS1))

Alternative Name: SOS1

Background: SOS1 antibody detects Son of sevenless homolog 1, a guanine nucleotide exchange factor (GEF) that activates Ras and Rac GTPases, serving as a critical link between receptor tyrosine kinases and downstream signaling cascades. SOS1 catalyzes the exchange of GDP for GTP on Ras, thereby triggering MAPK and PI3K pathway activation. The SOS1 antibody is widely used in cell signaling, oncology, and developmental biology to study Ras-mediated signal transduction and growth control.

SOS1 is encoded by the SOS1 gene on human chromosome 2p22.1. The protein is approximately 1333 amino acids long and consists of several domains: an N-terminal histone-like fold, a Dbl homology (DH) domain, a pleckstrin homology (PH) domain, and a C-terminal catalytic region responsible for Ras activation. SOS1 is recruited to the plasma membrane through interaction with adaptor proteins such as GRB2 following growth factor stimulation.

The SOS1 antibody detects a 155 kilodalton protein in western blot assays and shows cytoplasmic and membrane localization by immunofluorescence. Upon receptor activation, SOS1 facilitates Ras activation at the plasma membrane, leading to phosphorylation of ERK and other downstream targets. Through its DH-PH domains, SOS1 also activates Rac, linking actin cytoskeleton remodeling to growth factor signaling.

SOS1 plays essential roles in development, cell proliferation, and differentiation. Germline mutations in SOS1 cause Noonan syndrome, a developmental disorder characterized by short stature and congenital heart defects. In cancer, overexpression or dysregulation of SOS1 contributes to aberrant Ras activation and uncontrolled growth signaling. Its activity is finely tuned by feedback loops involving phosphorylation, ubiquitination, and membrane recruitment dynamics.

Because of its central position in the Ras signaling cascade, SOS1 is a valuable target for understanding receptor-driven proliferation and oncogenic transformation. NSJ Bioreagents provides a validated SOS1 antibody optimized for its applications, supporting research into Ras activation, MAPK pathway signaling, and cell differentiation control.

UniProt: Q07889

Pathways: RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling