TMEM9 antibody (AA 18-183)

Catalog No. ABIN7871397

The Rabbit Polyclonal anti-TMEM9 antibody is suitable to detect TMEM9 in samples from Human, Mouse and Rat. It has been validated for ELISA, WB and FACS.

Quick Overview for TMEM9 antibody (AA 18-183) (ABIN7871397)

Target: TMEM9 (Transmembrane Protein 9 (TMEM9))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TMEM9 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-TMEM9 Antibody

Binding Specificity: AA 18-183

Purpose: TMEM9 Antibody / Transmembrane protein 9

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human TMEM9 recombinant protein (Position: A18-S183) was used as the immunogen for the TMEM9 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TMEM9 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the TMEM9 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for TMEM9

Target: TMEM9 (Transmembrane Protein 9 (TMEM9))

Alternative Name: TMEM9

Background: TMEM9 antibody detects Transmembrane protein 9, encoded by the TMEM9 gene on chromosome 1q41. TMEM9 antibody is widely used in research on vesicle trafficking, lysosomal biology, and cancer. TMEM9 is a small lysosomal transmembrane protein that functions as a regulator of vesicle acidification and lysosomal activity. It contributes to autophagy, protein degradation, and receptor recycling, making it important for cell metabolism and signaling.

Structurally, TMEM9 is a ~21 kDa protein predicted to contain multiple transmembrane domains. It localizes to late endosomes and lysosomes, where it interacts with vacuolar H+-ATPases (V-ATPases) to promote acidification of the organelle lumen. This acidification is required for hydrolytic enzyme activity, autophagosome maturation, and lysosomal degradation. TMEM9 is conserved across vertebrates and is essential for maintaining lysosomal homeostasis.

Functionally, TMEM9 promotes lysosomal degradation and autophagy by enhancing V-ATPase assembly. It also regulates receptor recycling and endosomal trafficking. Dysregulation of TMEM9 disrupts lysosomal activity, leading to accumulation of undegraded proteins and impaired autophagy. Researchers use TMEM9 antibody to study vesicle trafficking, lysosomal acidification, and autophagy regulation.

Clinically, TMEM9 has been linked to cancer progression. Overexpression of TMEM9 has been reported in colorectal and liver cancers, where it promotes tumor growth by enhancing Wnt/beta-catenin signaling and metabolic adaptation. It may serve as a biomarker of aggressive tumor behavior and a potential therapeutic target. NSJ Bioreagents provides TMEM9 antibody for lysosomal, cancer, and metabolism studies.

Experimentally, TMEM9 antibody is applied in western blotting to detect the ~21 kDa protein, in immunofluorescence to study lysosomal localization, and in immunohistochemistry to assess tissue-specific expression. Co-immunoprecipitation with TMEM9 antibody helps identify interactions with V-ATPase components.

UniProt: Q9P0T7