FBXO7 antibody (AA 18-522)

Catalog No. ABIN7871427

The Rabbit Polyclonal anti-FBXO7 antibody is suitable to detect FBXO7 in samples from Human and Rat. It has been validated for WB, ELISA, IHC, IF, IP, ICC and FACS.

Quick Overview for FBXO7 antibody (AA 18-522) (ABIN7871427)

Target: FBXO7 (F-Box Protein 7 (FBXO7))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXO7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-FBXO7 Antibody

Binding Specificity: AA 18-522

Purpose: FBXO7 Antibody / F-box only protein 7

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human FBXO7 recombinant protein (Position: E18-M522) was used as the immunogen for the FBXO7 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the FBXO7 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the FBXO7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for FBXO7

Target: FBXO7 (F-Box Protein 7 (FBXO7))

Alternative Name: FBXO7

Background: FBXO7 antibody detects F-box only protein 7, an E3 ubiquitin ligase component that regulates protein degradation, cell cycle progression, and mitochondrial quality control. The UniProt recommended name is F-box only protein 7 (FBXO7). This protein belongs to the F-box family, which mediates substrate recognition within SCF (SKP1-CUL1-F-box) ubiquitin ligase complexes, promoting selective proteasomal degradation of target proteins.

Functionally, FBXO7 antibody identifies a 522-amino-acid cytoplasmic and nuclear protein containing an F-box domain for SCF complex binding and a proline-rich region that mediates protein-protein interactions. FBXO7 participates in cell cycle regulation by targeting cell cycle inhibitors for ubiquitination. It also plays a role in mitophagy by interacting with PINK1 and Parkin to promote the clearance of damaged mitochondria, maintaining neuronal and muscular health.

The FBXO7 gene is located on chromosome 22q12.3 and is broadly expressed, with high levels in brain, skeletal muscle, and lymphoid tissues. Its cellular distribution reflects multifunctional roles in ubiquitin-mediated proteolysis, cytoskeletal organization, and signal transduction.

Pathologically, mutations in FBXO7 are associated with Parkinsonian-pyramidal syndrome (PARK15), a neurodegenerative disorder combining parkinsonism with upper motor neuron dysfunction. Loss of FBXO7 disrupts mitophagy, leading to mitochondrial dysfunction and neurodegeneration. Research using FBXO7 antibody supports studies in protein degradation, neurobiology, and mitochondrial homeostasis.

FBXO7 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect F-box proteins and ubiquitination regulators. NSJ Bioreagents provides FBXO7 antibody reagents optimized for studies in proteostasis, neurodegeneration, and cell cycle control.

Structurally, F-box only protein 7 contains an F-box domain for SKP1 binding, coiled-coil regions for dimerization, and a ubiquitin-like domain that facilitates substrate recruitment. This antibody enables detailed characterization of FBXO7's function in E3 ligase activity and mitochondrial quality control.

UniProt: Q9Y3I1