EFS antibody (AA 183-553)

Catalog No. ABIN7871470

The Rabbit Polyclonal anti-EFS antibody (ABIN7871470) specifically detects EFS in WB, ELISA, IP and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for EFS antibody (AA 183-553) (ABIN7871470)

Target: EFS (Embryonal Fyn-Associated Substrate (EFS))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EFS antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-EFS Antibody

Binding Specificity: AA 183-553

Purpose: EFS Antibody / Embryonal Fyn-associated substrate

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human EFS recombinant protein (Position: E183-T553) was used as the immunogen for the EFS antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the EFS antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the EFS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for EFS

Target: EFS (Embryonal Fyn-Associated Substrate (EFS))

Alternative Name: EFS

Background: EFS antibody detects Embryonal Fyn-associated substrate, encoded by the EFS gene on chromosome 14q11.2. EFS antibody is commonly applied in studies of cytoskeletal organization, adhesion, and signaling. EFS belongs to the CAS (Crk-associated substrate) family of adaptor proteins, which includes BCAR1 (p130Cas) and NEDD9. It is predominantly expressed in embryonic tissues, endothelial cells, and hematopoietic cells, where it mediates signaling downstream of integrins, growth factors, and tyrosine kinases. Its discovery as a substrate of Fyn kinase highlighted its role in Src family kinase signaling networks.

Structurally, EFS contains an N-terminal SH3 domain, a substrate domain with multiple tyrosine phosphorylation sites, and a C-terminal focal adhesion targeting region. These domains allow EFS to interact with integrins, kinases, and actin cytoskeleton regulators. Phosphorylation of tyrosine residues by Src family kinases creates binding sites for SH2-containing adaptors such as Crk, linking EFS to downstream signaling cascades controlling adhesion and migration.

Functionally, EFS regulates integrin-mediated adhesion, migration, and cytoskeletal remodeling. It acts as a molecular scaffold at focal adhesions, coupling mechanical signals to intracellular pathways. EFS also participates in growth factor signaling, coordinating responses to PDGF, EGF, and VEGF. Knockdown of EFS reduces migration and disrupts adhesion dynamics, underscoring its importance in cell motility. Researchers employ EFS antibody to investigate focal adhesion signaling, cytoskeletal dynamics, and integrin biology.

Clinically, EFS has been linked to cancer, where altered expression contributes to invasion and metastasis. Its phosphorylation status serves as a marker of Src family kinase activity. In leukemia, EFS participates in abnormal signaling pathways that promote proliferation. EFS has also been studied in cardiovascular biology, where it regulates endothelial cell adhesion and angiogenesis. NSJ Bioreagents offers EFS antibody for use in oncology, vascular biology, and cell signaling research.

Experimentally, EFS antibody is applied in western blotting to detect the ~78 kDa protein, in immunohistochemistry to evaluate tissue distribution, and in immunofluorescence microscopy to visualize focal adhesion localization. Immunoprecipitation with EFS antibody enables analysis of phosphorylation status and identification of binding partners.

UniProt: O43281