UBE4B antibody (AA 184-1302)

Catalog No. ABIN7871473

The Rabbit Polyclonal anti-UBE4B antibody (ABIN7871473) specifically detects UBE4B in WB, ELISA, IF, ICC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for UBE4B antibody (AA 184-1302) (ABIN7871473)

Target: UBE4B (Ubiquitin Conjugation Factor E4 B (UBE4B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE4B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-UBE4B Antibody

Binding Specificity: AA 184-1302

Purpose: UBE4B Antibody / Ubiquitin conjugation factor E4 B

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human UBE4B recombinant protein (Position: S184-H1302) was used as the immunogen for the UBE4B antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the UBE4B antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the UBE4B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for UBE4B

Target: UBE4B (Ubiquitin Conjugation Factor E4 B (UBE4B))

Alternative Name: UBE4B

Background: UBE4B antibody detects Ubiquitin conjugation factor E4 B, encoded by the UBE4B gene. Ubiquitin conjugation factor E4 B is an E3/E4 hybrid ubiquitin ligase that regulates polyubiquitination of target proteins, contributing to protein turnover, quality control, and signaling regulation. UBE4B antibody provides researchers with a powerful reagent for studying ubiquitin-mediated degradation, proteostasis, and cellular signaling pathways.

Ubiquitin conjugation factor E4 B belongs to the U-box containing E3 ligase family and shares functional similarity with its yeast ortholog Ufd2. Research using UBE4B antibody has shown that it elongates ubiquitin chains initiated by E1 and E2 enzymes, ensuring effective targeting of substrates for proteasomal degradation. This E4 activity distinguishes UBE4B from classical E3 ligases and highlights its specialized function in ubiquitin chain extension.

Studies with UBE4B antibody have revealed its role in regulating key proteins involved in cell cycle, apoptosis, and DNA damage responses. UBE4B interacts with tumor suppressor p53 and facilitates its ubiquitination and degradation, thus modulating p53-dependent cell cycle arrest and apoptosis. Dysregulation of this process impacts oncogenesis, where UBE4B may act as both a regulator of survival and a potential therapeutic target.

Dysfunction of UBE4B has been associated with neurological disease and cancer. Research using UBE4B antibody has shown that abnormal activity contributes to neurodegeneration by disrupting protein quality control, while overexpression in cancer supports uncontrolled proliferation. These dual roles emphasize the importance of balanced ubiquitin regulation for normal physiology.

UBE4B antibody is commonly used in western blotting, immunoprecipitation, and immunohistochemistry. Western blotting quantifies UBE4B levels in tissues and cell lines, immunoprecipitation identifies ubiquitination targets, and immunohistochemistry demonstrates expression in tumors and brain regions. These applications make UBE4B antibody indispensable for proteostasis and disease research.

By providing validated UBE4B antibody reagents, NSJ Bioreagents supports studies into ubiquitination, protein degradation, and disease mechanisms. Detection of Ubiquitin conjugation factor E4 B provides researchers with insight into how ubiquitin ligases regulate cellular survival and stability.

UniProt: O95155