RBM12B antibody (AA 186-997)

Catalog No. ABIN7871511

The Rabbit Polyclonal anti-RBM12B antibody is suitable to detect RBM12B in samples from Human. It has been validated for WB, ELISA, FACS, ICC and IF.

Quick Overview for RBM12B antibody (AA 186-997) (ABIN7871511)

Target: RBM12B (RNA Binding Motif Protein 12B (RBM12B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBM12B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-RBM12B Antibody

Binding Specificity: AA 186-997

Purpose: RBM12B Antibody / RNA-binding protein 12B

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human RBM12B recombinant protein (Position: L186-K997) was used as the immunogen for the RBM12B antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the RBM12B antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the RBM12B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for RBM12B

Target: RBM12B (RNA Binding Motif Protein 12B (RBM12B))

Alternative Name: RBM12B

Background: RBM12B antibody detects RNA-binding protein 12B, a member of the RNA recognition motif (RRM) family that plays key roles in post-transcriptional gene regulation, mRNA processing, and nuclear RNA metabolism. RBM12B is believed to participate in alternative splicing and transcript stability by binding specific RNA motifs, thereby influencing gene expression patterns across multiple tissues. The RBM12B antibody is widely used in molecular biology and RNA research to study nuclear RNA dynamics, splicing regulation, and transcriptional coupling.

RBM12B is encoded by the RBM12B gene located on human chromosome 8q24.23. The protein is approximately 1,010 amino acids long and contains multiple RRM domains, each recognizing short RNA sequence elements. These RRMs are supported by intrinsically disordered regions that enable flexible interactions with other RNA-binding proteins and components of the spliceosome. RBM12B localizes primarily to the nucleus, where it forms speckle-like foci typical of splicing regulators.

The RBM12B antibody detects a 110-130 kilodalton band by western blot and reveals nuclear punctate staining under immunofluorescence microscopy. RBM12B interacts with heterogeneous nuclear ribonucleoproteins (hnRNPs) and splicing factors such as SRSF1, influencing exon inclusion and mRNA diversity. Through these interactions, RBM12B contributes to fine-tuning of transcript variants essential for cell type-specific expression and developmental processes.

Recent studies suggest RBM12B modulates gene expression linked to neuronal development and stress responses. Altered RBM12B expression has been reported in neurological disorders and cancer, where it affects transcriptome remodeling and mRNA turnover. Its paralog, RBM12, shares structural similarities, yet RBM12B may regulate distinct subsets of mRNAs, suggesting functional divergence within the family.

As a multifunctional RNA-binding protein, RBM12B connects transcriptional output with RNA processing and export. NSJ Bioreagents provides a validated RBM12B antibody optimized for its applications, supporting research into mRNA processing, splicing regulation, and gene expression control.

UniProt: Q8IXT5