STAMBP antibody (AA 187-424)

Catalog No. ABIN7871512

The Rabbit Polyclonal anti-STAMBP antibody (ABIN7871512) specifically detects STAMBP in WB, ELISA, IHC, ICC, IF and FACS. The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for STAMBP antibody (AA 187-424) (ABIN7871512)

Target: STAMBP (STAM Binding Protein (STAMBP))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAMBP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-STAMBP Antibody

Binding Specificity: AA 187-424

Purpose: STAMBP Antibody / STAM-binding protein

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human STAMBP recombinant protein (Position: K187-R424) was used as the immunogen for the STAMBP antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the STAMBP antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the STAMBP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for STAMBP

Target: STAMBP (STAM Binding Protein (STAMBP))

Alternative Name: STAMBP

Background: STAMBP antibody detects STAM-binding protein, a deubiquitinating enzyme that regulates endosomal sorting, cytokine receptor trafficking, and signal termination. Encoded by the STAMBP gene on chromosome 2p13.1, this enzyme is a member of the JAMM (JAB1/MPN/Mov34 metalloenzyme) family of zinc-dependent deubiquitinases that cleave ubiquitin chains from endosomal cargo proteins. STAMBP plays a critical role in maintaining receptor turnover and preventing abnormal accumulation of ubiquitinated proteins within endosomes.

Structurally, STAMBP is a 424-amino-acid cytoplasmic protein of approximately 49 kilodaltons containing a JAMM motif responsible for zinc ion coordination and catalytic activity, as well as SH3-binding regions that enable interaction with signal transducing adaptor molecules (STAM1 and STAM2). It localizes to early endosomes and participates in the endosomal sorting complex required for transport (ESCRT) machinery, modulating trafficking of growth factor receptors such as EGFR and cytokine receptors after ligand binding.

The STAMBP antibody is widely used in cell signaling, membrane trafficking, and neurodevelopmental research to study ubiquitin processing, receptor regulation, and lysosomal degradation pathways. Western blot analysis detects a 49 kilodalton band corresponding to STAMBP, while immunofluorescence reveals punctate endosomal staining. This antibody enables precise detection of endosomal deubiquitinating enzyme activity and ESCRT complex assembly.

Functionally, STAMBP removes Lys63-linked ubiquitin chains from endosomal cargo proteins, facilitating recycling or degradation decisions within the endolysosomal pathway. Its activity prevents overactivation of growth factor signaling and supports normal receptor turnover. Mutations in STAMBP cause microcephaly-capillary malformation syndrome (MIC-CAP), a rare congenital disorder characterized by neurodevelopmental delay, seizures, and vascular malformations resulting from defective ubiquitin recycling. Altered STAMBP expression also contributes to tumor progression and immune dysregulation through prolonged receptor signaling. The STAMBP antibody provides a powerful tool for characterizing ubiquitin-dependent trafficking, growth factor regulation, and endosomal sorting mechanisms. NSJ Bioreagents validates this antibody for its applications, ensuring robust and reproducible detection in trafficking and signaling studies.

UniProt: O95630