PHTF1 antibody (AA 188-430)

Catalog No. ABIN7871517

The Rabbit Polyclonal anti-PHTF1 antibody is suitable to detect PHTF1 in samples from Human. It has been validated for WB, ELISA and FACS.

Quick Overview for PHTF1 antibody (AA 188-430) (ABIN7871517)

Target: PHTF1 (Putative Homeodomain Transcription Factor 1 (PHTF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHTF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-PHTF1 Antibody

Binding Specificity: AA 188-430

Purpose: PHTF1 Antibody / Putative homeodomain transcription factor 1

Purification: Antigen affinity purified

Immunogen: E. coli-derived recombinant human protein (amino acids I188-Q430) was used as the immunogen for the PHTF1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PHTF1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PHTF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PHTF1

Target: PHTF1 (Putative Homeodomain Transcription Factor 1 (PHTF1))

Alternative Name: PHTF1

Background: PHTF1 (putative homeodomain transcription factor 1), also known as PHTF, is a potential transcription regulator. It is a ubiquitously expressed integral, multipass membrane protein with predominant expression in testis. PHTF1 is associated with the ER (endoplasmic reticulum) and contains one bHLH (basic helix-loop-helix) domain. It is present in the cell during meiosis and spermiogenesis but, by the end of spermiogenesis, is released from the mature cell within the residual bodies. This implies that PHTF1 may play a role in the spermatozoa maturation process. In addition, PHTF1 is believed to interact with FEM1B and may be responsible for recruiting FEM1B to the surface of the ER membrane. This suggests that PHTF1 acts as a sequestering or anchoring protein for FEM1B. Two PHTF1 isoforms exist due to alternate splicing events. Isoform 2 is the shorter form and lacks the amino acid residues 648 to 762.

UniProt: Q9UMS5