FSHB antibody (AA 19-130)

Catalog No. ABIN7871556

The Rabbit Polyclonal anti-FSHB antibody is suitable to detect FSHB in samples from Zebrafish (Danio rerio). It has been validated for WB and IHC (p).

Quick Overview for FSHB antibody (AA 19-130) (ABIN7871556)

Target: FSHB (Follicle Stimulating Hormone, beta Polypeptide (FSHB))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FSHB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FSHB Antibody

Binding Specificity: AA 19-130

Purpose: Zebrafish Fshb Antibody / Follicle stimulating hormone beta

Purification: Antigen affinity chromatography

Immunogen: E. coli-derived zebrafish Fshb recombinant protein (amino acids E19-H130) was used as the immunogen for the Zebrafish Fshb antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal dilution of the Zebrafish Fshb antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Zebrafish Fshb antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for FSHB

Target: FSHB (Follicle Stimulating Hormone, beta Polypeptide (FSHB))

Alternative Name: Fshb

Background: Follicle-stimulating hormone (FSH) is a key glycoprotein hormone involved in the regulation of reproductive processes across vertebrate species. It is composed of a common alpha subunit and a hormone-specific beta subunit, with the beta subunit (Fshb) conferring biological specificity. In zebrafish (Danio rerio), the fshb gene encodes the beta subunit of FSH and is expressed in gonadotrope cells of the anterior pituitary.

Zebrafish Fshb plays a crucial role in the development and maturation of gametes, particularly in promoting folliculogenesis in females and spermatogonial proliferation in males. Its expression and secretion are primarily regulated by gonadotropin-releasing hormone (GnRH) and modulated by feedback from sex steroids and other endocrine factors within the hypothalamic-pituitary-gonadal (HPG) axis.

During zebrafish development, fshb expression becomes evident post-embryonically and increases with sexual maturation. Functional studies have shown that Fshb is essential for early-stage follicle development and the onset of puberty, while also contributing to basal steroidogenesis in the gonads.

Given the genetic accessibility and transparency of zebrafish, the fshb gene and its protein product are commonly used in reproductive biology, toxicology, and endocrine disruption studies. Zebrafish Fshb shares structural and functional homology with its mammalian counterparts, making it a valuable model for exploring conserved mechanisms of vertebrate reproduction.

UniProt: Q6TCF7

Pathways: Peptide Hormone Metabolism, Hormone Activity, C21-Steroid Hormone Metabolic Process