LYZ antibody (AA 19-148)

Catalog No. ABIN7871560

The Rabbit Polyclonal anti-LYZ antibody (ABIN7871560) specifically detects LYZ in WB, IHC and ELISA. The antibody is reactive with Mouse and Rat samples.

Quick Overview for LYZ antibody (AA 19-148) (ABIN7871560)

Target: LYZ (Lysozyme (LYZ))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LYZ antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-LYZ Antibody

Binding Specificity: AA 19-148

Purpose: Lysozyme Antibody / Lyz1 / Lyz2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived mouse Lyz1/2 recombinant protein (Position: K19-V148) was used as the immunogen for the Lysozyme antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Lysozyme antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Lysozyme antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for LYZ

Target: LYZ (Lysozyme (LYZ))

Alternative Name: Lysozyme

Background: Lysozyme antibody detects Lysozyme, encoded by the LYZ gene in human and by two closely related genes, Lyz1 and Lyz2, in mouse. Lysozyme is a secreted enzyme that cleaves bacterial peptidoglycan, serving as a critical effector of innate immunity. Lysozyme antibody provides researchers with a widely used reagent for studying antimicrobial defense, innate immune responses, and myeloid cell biology.

Lysozyme is one of the most abundant proteins in neutrophils, macrophages, and secretions such as saliva, tears, and mucus. Research using Lysozyme antibody has shown that it hydrolyzes the beta-1,4-glycosidic bonds in bacterial cell walls, leading to lysis of Gram-positive bacteria and contributing to host defense. This antimicrobial activity makes Lysozyme a frontline defense factor in mucosal immunity.

Studies with Lysozyme antibody have revealed that beyond antimicrobial activity, Lysozyme contributes to modulation of inflammation. It regulates cytokine release, scavenges bacterial products, and influences macrophage polarization. This dual antimicrobial and immunomodulatory role underscores its importance in both acute infection and chronic inflammation.

Dysregulation of Lysozyme has been linked to disease. Research using Lysozyme antibody has shown that mutations in the human LYZ gene can cause hereditary systemic amyloidosis, characterized by Lysozyme amyloid deposits in the kidney, liver, and gastrointestinal tract. Elevated Lysozyme expression has been observed in leukemia and inflammatory disorders, where it can serve as a biomarker of disease activity. Mouse models using Lyz1 and Lyz2 further highlight the importance of Lysozyme in host-pathogen interactions.

Lysozyme antibody is commonly used in immunohistochemistry, immunofluorescence, and western blotting. Immunohistochemistry highlights myeloid cell populations in tissue sections, immunofluorescence demonstrates localization in macrophages and neutrophils, and western blotting quantifies expression across tissues and disease models. These applications make Lysozyme antibody indispensable in immunology and infection research.

By providing validated Lysozyme antibody reagents, NSJ Bioreagents supports studies into host defense, innate immunity, and disease. Detection of Lysozyme provides researchers with insight into how antimicrobial enzymes shape immunity and pathology.

UniProt: P17897