MATN4 antibody (AA 191-590)

Catalog No. ABIN7871670

The Rabbit Polyclonal anti-MATN4 antibody is suitable to detect MATN4 in samples from Human. It has been validated for ELISA, WB and FACS.

Quick Overview for MATN4 antibody (AA 191-590) (ABIN7871670)

Target: MATN4 (Matrilin 4 (MATN4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MATN4 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-MATN4 Antibody

Binding Specificity: AA 191-590

Purpose: MATN4 Antibody / Matrilin 4

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human Matrilin-4/MATN4 recombinant protein (Position: E191-Q590) was used as the immunogen for the MATN4 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MATN4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MATN4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MATN4

Target: MATN4 (Matrilin 4 (MATN4))

Alternative Name: MATN4

Background: MATN4 antibody detects Matrilin-4, a secreted extracellular matrix adapter protein that contributes to the structural organization of cartilage, tendon, and connective tissue matrices. The UniProt recommended name is Matrilin-4 (MATN4). This glycoprotein is a member of the matrilin family, which functions as extracellular linkers connecting collagen fibrils, proteoglycans, and other matrix molecules.

Functionally, MATN4 antibody identifies a 602-amino-acid modular protein containing von Willebrand factor A (vWFA) domains and coiled-coil motifs. MATN4 interacts with matrix components such as aggrecan, COMP, and collagens II and IX, facilitating the formation of filamentous networks that provide tensile strength and elasticity to connective tissues. It plays a structural and regulatory role in cartilage organization and tissue resilience under mechanical load.

The MATN4 gene is located on chromosome 20q13.12 and encodes a protein expressed primarily in skeletal tissues, including cartilage, intervertebral discs, and developing bone. Matrilin-4 functions as an adaptor molecule linking fibrillar collagens to proteoglycan aggregates, stabilizing the extracellular matrix architecture. During development, MATN4 expression is tightly regulated by transcription factors such as SOX9 and RUNX2 that control chondrogenesis and osteogenesis.

In physiology, MATN4 supports cartilage integrity and joint function. Reduced expression or mutations in matrilin family proteins can impair cartilage matrix stability, predisposing to osteoarthritis or connective tissue degeneration. MATN4 also contributes to tissue repair by enhancing chondrocyte adhesion and matrix assembly following injury. In cancer biology, altered MATN4 expression has been reported in breast, gastric, and pancreatic cancers, where it may influence tumor cell adhesion and invasion through modulation of the extracellular matrix microenvironment.

MATN4 antibody is widely used in extracellular matrix, musculoskeletal, and developmental biology research. It is suitable for immunohistochemistry, ELISA, and western blotting to detect Matrilin-4 expression and distribution. This antibody supports studies of cartilage formation, joint pathology, and matrix protein interactions. In biomaterials research, MATN4 serves as a reference marker for cartilage scaffold design and regenerative tissue engineering.

Structurally, Matrilin-4 consists of vWFA domains that mediate protein-protein binding and a coiled-coil domain enabling tetramer formation. It is secreted via the classical ER-Golgi pathway and undergoes glycosylation for structural stability. NSJ Bioreagents provides MATN4 antibody reagents validated for use in extracellular matrix organization, skeletal development, and tissue engineering research.

UniProt: O95460