MICAL-Like 1 antibody (AA 192-747)

Catalog No. ABIN7871677

The Rabbit Polyclonal anti-MICAL-Like 1 antibody (ABIN7871677) specifically detects MICAL-Like 1 in WB, IHC, ELISA and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MICAL-Like 1 antibody (AA 192-747) (ABIN7871677)

Target: MICAL-Like 1 (MICALL1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MICAL-Like 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-MICAL-Like 1 Antibody

Binding Specificity: AA 192-747

Purpose: MICALL1 Antibody / MICAL-like 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human MICALL1 recombinant protein (Position: R192-K747) was used as the immunogen for the MICALL1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MICALL1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MICALL1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MICAL-Like 1

Target: MICAL-Like 1 (MICALL1)

Alternative Name: MICALL1

Background: MICALL1 antibody detects MICAL-like protein 1, a cytoskeletal and membrane remodeling protein that regulates endocytic recycling and actin dynamics. MICALL1 belongs to the MICAL family of flavoprotein monooxygenases and adaptors that control trafficking of surface receptors and junctional complexes. Unlike enzymatically active MICALs, MICALL1 acts primarily as a scaffold, coordinating interactions between small GTPases and cytoskeletal components. The MICALL1 antibody is widely used to study vesicular trafficking, recycling endosomes, and cellular polarity.

MICALL1 is encoded by the MICALL1 gene located on human chromosome 22q13.1. The protein is approximately 94 kilodaltons and includes a calponin homology (CH) domain, a LIM domain, and multiple coiled-coil regions that mediate binding to Rab GTPases and the EHD family of endocytic regulators. MICALL1 localizes to tubular recycling endosomes, where it coordinates membrane curvature and receptor sorting.

The MICALL1 antibody detects a 94 kilodalton protein by western blot and shows punctate cytoplasmic staining in immunofluorescence. MICALL1 interacts with Rab8, Rab11, and EHD1, forming a complex that facilitates recycling of receptors such as transferrin and integrins to the plasma membrane. It also binds to actin regulatory proteins, linking vesicle trafficking to cytoskeletal rearrangements and cell morphology maintenance.

Disruption of MICALL1 expression impairs membrane recycling and causes accumulation of endosomal tubules. Mutations in MICALL1 are associated with autosomal recessive neurodevelopmental disorders characterized by ataxia and delayed myelination. In epithelial cells, MICALL1 participates in maintaining tight junction integrity and apical-basal polarity, processes essential for tissue organization.

Because of its central role in coordinating membrane transport, MICALL1 contributes to diverse biological pathways, from nutrient uptake to synaptic vesicle recycling. NSJ Bioreagents provides a validated MICALL1 antibody optimized for its applications, supporting research into vesicular dynamics, receptor trafficking, and cell polarity mechanisms.

UniProt: Q8N3F8