SETD5 antibody (AA 196-1275)

Catalog No. ABIN7871707

The Rabbit Polyclonal anti-SETD5 antibody (ABIN7871707) specifically detects SETD5 in ELISA, WB and FACS. The antibody is reactive with Human samples.

Quick Overview for SETD5 antibody (AA 196-1275) (ABIN7871707)

Target: SETD5 (SET Domain Containing 5 (SETD5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SETD5 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-SETD5 Antibody

Binding Specificity: AA 196-1275

Purpose: SETD5 Antibody / SET domain-containing protein 5

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SETD5 recombinant protein (Position: Q196-R1275) was used as the immunogen for the SETD5 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SETD5 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SETD5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SETD5

Target: SETD5 (SET Domain Containing 5 (SETD5))

Alternative Name: SETD5

Background: SETD5 antibody detects SET domain-containing protein 5, a chromatin-associated enzyme implicated in epigenetic regulation, transcriptional control, and neurodevelopment. Encoded by the SETD5 gene on chromosome 3p25.3, this protein contains a conserved SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain characteristic of lysine methyltransferases. Although its precise enzymatic targets remain under investigation, SETD5 is thought to influence chromatin structure and gene accessibility by interacting with histone-modifying and transcriptional complexes.

Structurally, SETD5 is a large nuclear protein of approximately 1,442 amino acids and 170 kilodaltons containing an N-terminal SET domain and coiled-coil regions that mediate interactions with epigenetic cofactors such as HDAC3 and PAF1C. SETD5 localizes primarily to euchromatin, where it contributes to transcriptional repression and developmental gene regulation. It is broadly expressed, with highest levels in brain and embryonic tissues, supporting its essential role in differentiation and neurogenesis.

The SETD5 antibody is widely used in epigenetics, neuroscience, and developmental biology research to study chromatin remodeling, gene silencing, and neurodevelopmental disorders. Western blot analysis detects a 170 kilodalton band corresponding to SETD5, while immunofluorescence demonstrates nuclear localization with punctate chromatin-associated staining. This antibody provides a reliable reagent for assessing epigenetic enzyme expression and chromatin regulatory function.

Loss-of-function mutations in SETD5 are associated with autosomal dominant intellectual disability and developmental delay syndromes. Functional studies show that SETD5 regulates genes involved in cell cycle control, neural differentiation, and synapse formation through coordination of histone deacetylation and RNA polymerase II elongation. It acts as an integrator of epigenetic and transcriptional machinery, maintaining proper expression of developmental regulators. Dysregulated SETD5 expression has been linked to tumor progression, particularly in cancers with altered chromatin modification landscapes. The SETD5 antibody provides an essential tool for exploring the interplay between chromatin dynamics, transcriptional control, and development. NSJ Bioreagents validates this antibody for its applications, ensuring dependable performance in epigenetic research.

UniProt: Q9C0A6