XAF1 antibody (AA 197-301)

Catalog No. ABIN7871745

The Rabbit Polyclonal anti-XAF1 antibody is suitable to detect XAF1 in samples from Human. It has been validated for WB, ELISA, IHC (p) and FACS.

Quick Overview for XAF1 antibody (AA 197-301) (ABIN7871745)

Target: XAF1 (XiAP Associated Factor 1 (XAF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XAF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Product Details anti-XAF1 Antibody

Binding Specificity: AA 197-301

Purpose: XIAP associated factor 1 Antibody / XAF1

Purification: Antigen affinity purified

Immunogen: Recombinant human protein (amino acids Q197-S301) was used as the immunogen for the XIAP associated factor 1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the XIAP associated factor 1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the XIAP associated factor 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for XAF1

Target: XAF1 (XiAP Associated Factor 1 (XAF1))

Alternative Name: XIAP associated factor 1

Background: XAF1, also called XIAP-associated factor 1, antagonizes the anti-caspase activity of XIAP and may be important in mediating apoptosis resistance in cancer cells. By genomic sequence analysis, the XAF1 gene contains 7 exons spanning 18 kb. Southern blot analysis suggested that XAF1 is a single-copy gene. The XAF1 gene is mapped on 17p13.1. Fluorescence microscopy demonstrated nuclear expression of XAF1, in contrast to the cytoplasmic expression of XIAP. Functional analysis indicated that XAF1 reverses the XIAP-mediated inhibition of CASP3 and its protection against apoptosis. Northern blot analysis detected wide expression of 3.9-, 4.5-, 6.0-, and 7.0-kb XAF1 transcripts, with highest levels in heart and ovary and lowest levels in brain and testis. Antisense experiments determined that loss of XAF1 expression enhances resistance to apoptosis. Alteration in XAF1 and XIAP RNA expression levels may lead to increased apoptotic resistance and proliferation due to unregulated XIAP function in cancer cells.

UniProt: Q6GPH4