PKN3 antibody (AA 2-889)

Catalog No. ABIN7871827

The Rabbit Polyclonal anti-PKN3 antibody (ABIN7871827) specifically detects PKN3 in WB, ELISA and FACS. The antibody is reactive with Human samples.

Quick Overview for PKN3 antibody (AA 2-889) (ABIN7871827)

Target: PKN3 (Protein Kinase N3 (PKN3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKN3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-PKN3 Antibody

Binding Specificity: AA 2-889

Purpose: PKN3 Antibody / Protein kinase N3

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human PKN3 recombinant protein (Position: E2-P889) was used as the immunogen for the PKN3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PKN3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PKN3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PKN3

Target: PKN3 (Protein Kinase N3 (PKN3))

Alternative Name: PKN3

Background: PKN3 antibody detects Protein kinase N3, a serine/threonine kinase involved in cytoskeletal organization, cell adhesion, and tumorigenesis. Encoded by the PKN3 gene on chromosome 9q31.2, this enzyme is a member of the protein kinase N (PKN) family, which are effectors of the Rho family of small GTPases. PKN3 links Rho signaling to downstream pathways that regulate actin dynamics, cell migration, and proliferation, making it a key mediator of cytoskeletal remodeling and oncogenic transformation.

Structurally, PKN3 contains an N-terminal regulatory region with HR1 (homology region 1) motifs that bind Rho GTPases, followed by a C-terminal catalytic domain similar to protein kinase C (PKC). PKN3 phosphorylates substrates involved in actin filament stabilization and cell junction assembly, coordinating mechanical and adhesive cues. It is localized to the cytoplasm and plasma membrane, where it interacts with focal adhesion proteins and scaffolds to transmit signals influencing motility and growth.

The PKN3 antibody is widely used in cancer biology, cell signaling, and cytoskeletal research to investigate Rho-dependent kinase pathways and cell morphology control. Western blot analysis detects a 110 kilodalton band corresponding to PKN3, while immunofluorescence shows diffuse cytoplasmic and membrane-associated staining in epithelial and tumor cells. This antibody supports research into the molecular basis of migration, invasion, and metastatic progression.

PKN3 is overexpressed in several cancers, including breast, prostate, and pancreatic carcinomas, where it promotes invasive behavior through activation of the PI3K/AKT and RhoA pathways. Genetic or pharmacologic inhibition of PKN3 suppresses tumor growth and metastasis, making it a promising therapeutic target. Beyond oncology, PKN3 also contributes to endothelial cell barrier function and angiogenesis. The PKN3 antibody provides a valuable reagent for dissecting the cellular functions and therapeutic potential of this kinase. NSJ Bioreagents validates this antibody for its applications, ensuring consistent and reliable results for signal transduction research.

UniProt: Q6P5Z2