NrCAM antibody (AA 207-1025)

Catalog No. ABIN7872106

The Rabbit Polyclonal anti-NrCAM antibody (ABIN7872106) specifically detects NrCAM in WB, ELISA and FACS. The antibody is reactive with Mouse and Rat samples.

Quick Overview for NrCAM antibody (AA 207-1025) (ABIN7872106)

Target: NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NrCAM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-NrCAM Antibody

Binding Specificity: AA 207-1025

Purpose: NRCAM Antibody / Neuronal cell adhesion molecule

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human NRCAM recombinant protein (Position: R207-Q1025) was used as the immunogen for the NRCAM antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NRCAM antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the NRCAM antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for NrCAM

Target: NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))

Alternative Name: NRCAM

Background: NRCAM antibody detects Neuronal cell adhesion molecule, a type I transmembrane glycoprotein belonging to the L1 family of cell adhesion molecules (CAMs). NRCAM promotes axonal guidance, synapse formation, and cell-cell communication during nervous system development. The NRCAM antibody is widely used in neuroscience, developmental biology, and neuro-oncology research to study neuronal adhesion, axonal fasciculation, and signal transduction.

NRCAM is encoded by the NRCAM gene located on human chromosome 7q31.1. The protein is approximately 135 kilodaltons and comprises six immunoglobulin-like domains, five fibronectin type III repeats, a single transmembrane segment, and a short cytoplasmic tail containing ankyrin-binding motifs. These structural features allow NRCAM to interact with both extracellular ligands and intracellular cytoskeletal adaptors, linking adhesion to signal transduction and cytoskeletal organization.

The NRCAM antibody detects a 135 kilodalton band by western blot and exhibits strong membrane and axonal staining in neuronal cultures and brain tissue. NRCAM mediates homophilic and heterophilic interactions with other cell adhesion molecules, including contactin and L1CAM, forming complexes that regulate neurite outgrowth, axonal pathfinding, and synapse stabilization. In developing neurons, NRCAM clusters at growth cones, coordinating adhesion with actin dynamics and microtubule extension.

NRCAM also participates in signaling cascades involving the MAPK and PI3K pathways, modulating neuronal survival and differentiation. In non-neuronal tissues, NRCAM contributes to cell migration and epithelial morphogenesis, while in cancer, its overexpression promotes tumor cell adhesion and metastasis, particularly in melanoma, glioma, and prostate carcinoma.

Genetic and expression studies link NRCAM variants to neurodevelopmental and psychiatric conditions, including autism spectrum disorder and schizophrenia, suggesting that altered cell adhesion signaling may underlie synaptic dysfunction. NSJ Bioreagents provides a validated NRCAM antibody optimized for its applications, enabling detailed investigation of neuronal adhesion, developmental patterning, and disease-related signaling mechanisms.

UniProt: Q810U4

Pathways: Regulation of Cell Size