TIM3 antibody (AA 22-202)

Catalog No. ABIN7872292

The Mouse Monoclonal anti-TIM3 antibody (Clone TIM3-4027) (ABIN7872292) specifically detects TIM3 in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for TIM3 antibody (AA 22-202) (ABIN7872292)

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIM3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: TIM3-4027

Product Details anti-TIM3 Antibody

Binding Specificity: AA 22-202

Purpose: TIM-3 Antibody / HAVCR2 (azide and preservative free)

Purification: Protein A/G affinity

Immunogen: A portion of amino acids 22-202 was used as the immunogen for the TIM-3 antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal dilution of the TIM-3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: -20 °C

Storage Comment: Aliquot the TIM-3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for TIM3

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Alternative Name: TIM-3

Background: TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. TIM-3 is expressed on the surface of effector T cell tonsil, lymph node or spleen. ls (CD4+Th1 and CD8+Tc1) but not on helper T cells (CD4+Th2 and CD8+Tc2). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells. The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and pro-apoptotic signaling. TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity, regulatory T cell development, and increases in macrophage and neutrophil infiltration into sites of inflammation.

UniProt: Q8TDQ0

Pathways: Regulation of Lipid Metabolism by PPARalpha, Cancer Immune Checkpoints