TIM3 antibody (AA 22-202)

Catalog No. ABIN7872307

The Mouse Monoclonal anti-TIM3 antibody (Clone TIM3-3113) (ABIN7872307) specifically detects TIM3 in IHC (p). The antibody is reactive with Human samples.

Quick Overview for TIM3 antibody (AA 22-202) (ABIN7872307)

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIM3 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: TIM3-3113

Product Details anti-TIM3 Antibody

Binding Specificity: AA 22-202

Purpose: TIM3 Antibody / HAVCR2

Purification: Protein G affinity chromatography

Immunogen: A recombinant fragment of human TIM3 protein within amino acids 22-202 was used as the immunogen for the TIM3 antibody.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal dilution of the TIM3 antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the TIM3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for TIM3

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Alternative Name: TIM3

Background: TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. TIM-3 is expressed on the surface of effector T cells (CD4+Th1 and CD8+Tc1) but not on helper T cells (CD4+Th2 and CD8+Tc2). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells. The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and pro-apoptotic signaling. TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity, regulatory T cell development, and increases in macrophage and neutrophil infiltration into sites of inflammation.

UniProt: Q8TDQ0

Pathways: Regulation of Lipid Metabolism by PPARalpha, Cancer Immune Checkpoints