MFSD2A antibody (AA 22-543)

Catalog No. ABIN7872330

The Rabbit Polyclonal anti-MFSD2A antibody is suitable to detect MFSD2A in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, IF and ICC.

Quick Overview for MFSD2A antibody (AA 22-543) (ABIN7872330)

Target: MFSD2A (Major Facilitator Superfamily Domain Containing 2A (MFSD2A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MFSD2A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-MFSD2A Antibody

Binding Specificity: AA 22-543

Purpose: MFSD2A Antibody / Major facilitator superfamily domain-containing protein 2A

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human MFSD2A recombinant protein (Position: Q22-L543) was used as the immunogen for the MFSD2A antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MFSD2A antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MFSD2A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MFSD2A

Target: MFSD2A (Major Facilitator Superfamily Domain Containing 2A (MFSD2A))

Alternative Name: MFSD2A

Background: MFSD2A antibody detects Major facilitator superfamily domain-containing protein 2A, a sodium-dependent lysophosphatidylcholine symporter that mediates the uptake of docosahexaenoic acid (DHA) into the brain and retina. MFSD2A plays a critical role in maintaining the blood-brain barrier (BBB) and enabling lipid transport across endothelial cells. The MFSD2A antibody is widely used in neuroscience, vascular biology, and metabolic research to investigate BBB integrity, lipid trafficking, and neurodevelopmental regulation.

MFSD2A is encoded by the MFSD2A gene located on human chromosome 1p34.2. The protein belongs to the major facilitator superfamily of solute carriers and is approximately 530 amino acids in length, with 12 predicted transmembrane helices. MFSD2A localizes to the plasma membrane of endothelial cells lining brain capillaries, where it functions as a transporter for lysophosphatidylcholine-bound polyunsaturated fatty acids. Unlike most nutrient transporters at the BBB, MFSD2A is not energy-dependent but couples lipid import to sodium gradients.

The MFSD2A antibody detects a 62 kilodalton band in western blot and demonstrates strong vascular endothelial staining in brain tissue and retina. In mouse models, deletion of MFSD2A results in severe microcephaly, neuronal loss, and impaired synaptic development due to reduced DHA delivery to the brain. In adults, MFSD2A maintains BBB tightness by suppressing vesicular transcytosis, ensuring selective permeability and protecting the central nervous system from toxins and immune cell infiltration.

MFSD2A expression is regulated by pericytes and Wnt/beta-catenin signaling pathways, linking vascular maturation to lipid metabolism. Its dysfunction has been associated with neurodevelopmental disorders, cognitive impairment, and visual defects. In cancer research, MFSD2A acts as a metastasis suppressor by inhibiting vascular leakage and tumor cell extravasation, demonstrating a dual role in nutrient transport and vascular homeostasis.

Beyond the nervous system, MFSD2A participates in lipid absorption in the placenta and peripheral vasculature. Because of its essential function in brain DHA uptake, MFSD2A is a promising target for therapeutic strategies aimed at enhancing neuroprotection and treating BBB disorders. NSJ Bioreagents provides a validated MFSD2A antibody optimized for western blot, immunohistochemistry, and confocal microscopy, supporting studies of lipid transport, barrier regulation, and neurovascular signaling.

UniProt: Q8NA29