NEK5 antibody (AA 22-627)

Catalog No. ABIN7872332

This Rabbit Polyclonal antibody specifically detects NEK5 in ELISA, WB, IHC, IF and ICC. It exhibits reactivity toward Mouse and Rat.

Quick Overview for NEK5 antibody (AA 22-627) (ABIN7872332)

Target: NEK5 (NIMA-Related Kinase 5 (NEK5))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK5 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-NEK5 Antibody

Binding Specificity: AA 22-627

Purpose: NEK5 Antibody / NimA-related protein kinase 5

Purification: Immunogen affinity purified

Immunogen: E.coli-derived mouse NEK5 recombinant protein (Position: K22-Q627) was used as the immunogen for the NEK5 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NEK5 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the NEK5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for NEK5

Target: NEK5 (NIMA-Related Kinase 5 (NEK5))

Alternative Name: NEK5

Background: The NEK5 antibody targets Serine/threonine-protein kinase Nek5, an enzyme encoded by the NEK5 gene and belonging to the NIMA-related kinase (NEK) family. These kinases regulate cell cycle progression, centrosome dynamics, and DNA damage responses. Serine/threonine-protein kinase Nek5 is implicated in maintaining mitochondrial integrity, regulating apoptosis, and coordinating mitotic entry. The NEK5 antibody provides a valuable reagent for studying kinase signaling networks that ensure genomic stability and cellular homeostasis.

Serine/threonine-protein kinase Nek5 contains an N-terminal catalytic domain with conserved motifs for ATP binding and phosphorylation activity, and a C-terminal region responsible for subcellular localization and protein interactions. It localizes to centrosomes and mitochondria, suggesting dual functions in cell division and energy metabolism. The NEK5 antibody supports studies of these distinct localizations, allowing researchers to examine how Nek5 activity integrates cell-cycle control with mitochondrial health. Evidence indicates that Nek5 contributes to the initiation of centrosome disjunction, ensuring proper spindle formation during mitosis.

Beyond its role in mitotic regulation, Serine/threonine-protein kinase Nek5 has emerged as a mediator of mitochondrial dynamics and apoptosis resistance. It stabilizes mitochondrial membrane potential and reduces reactive oxygen species accumulation, linking Nek5 function to cellular stress adaptation. The NEK5 antibody helps reveal how mitochondrial Nek5 signaling intersects with pathways involving BCL2 family proteins and caspase activation. Studies have suggested that loss of Nek5 increases sensitivity to oxidative damage and promotes apoptotic cell death, highlighting its potential importance in tissue homeostasis and neuroprotection.

Aberrant Nek5 expression or activity may contribute to cancer development and progression. Overexpression has been observed in several tumor types, including breast and prostate cancers, where it may promote survival under genotoxic stress. The NEK5 antibody supports exploration of these oncogenic associations, enabling analysis of protein levels, phosphorylation status, and subcellular distribution in tumor models. Functional studies continue to investigate Nek5 as a possible target for kinase inhibition strategies aimed at restoring mitotic fidelity and limiting tumor growth.

The NEK5 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry. It yields strong centrosomal and mitochondrial staining consistent with its dual localization. NSJ Bioreagents provides this antibody as a validated, high-specificity reagent for cellular and molecular biology research. By supporting detailed analysis of Serine/threonine-protein kinase Nek5 expression and signaling, the NEK5 antibody contributes to understanding of mitotic regulation, mitochondrial health, and cancer cell survival mechanisms.

UniProt: Q7TSC3

Pathways: Regulation of Actin Filament Polymerization