Splicing Factor 1 antibody (AA 220-461)

Catalog No. ABIN7872351

The Mouse Monoclonal anti-Splicing Factor 1 antibody is suitable to detect Splicing Factor 1 in samples from Human. It has been validated for IHC (p).

Quick Overview for Splicing Factor 1 antibody (AA 220-461) (ABIN7872351)

Target: Splicing Factor 1 (SF1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Splicing Factor 1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: NR5A1-3420

Product Details anti-Splicing Factor 1 Antibody

Binding Specificity: AA 220-461

Purpose: SF-1 Antibody / Steroidogenic Factor 1 / NR5A1

Purification: Protein A/G affinity

Immunogen: A portion of amino acids 220-461 was used as the immunogen for the SF-1 antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal dilution of the Steroidogenic Factor 1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Steroidogenic Factor 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for Splicing Factor 1

Target: Splicing Factor 1 (SF1)

Alternative Name: SF-1

Background: The protein encoded by this gene is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this gene are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect. Steroidogenic Factor 1 (SF-1) is considered an orphan nuclear receptor that belongs to subfamily 5. It was found to be a regulator of steroidogenic enzyme gene expression. Oxysterols are suggested as its ligands. It is expressed in all steroidogenic tissues, including the adrenal cortex, testicular Sertoli cells, and Leydig cells, ovarian theca, hypothalamus, and anterior pituitary. SF-1 plays an important role in adrenal and gonadal development. SF-1 is highly valuable marker to determine the adrenocortical origin of an adrenal mass.

UniProt: Q13285

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location