TSR1 antibody (AA 224-723)

Catalog No. ABIN7872395

This Rabbit Polyclonal antibody specifically detects TSR1 in ELISA, WB, IP, IF, FACS and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TSR1 antibody (AA 224-723) (ABIN7872395)

Target: TSR1 (TSR1, 20S rRNA Accumulation, Homolog (TSR1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TSR1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-TSR1 Antibody

Binding Specificity: AA 224-723

Purpose: TSR1 Antibody / Pre-rRNA-processing protein TSR1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human TSR1 recombinant protein (Position: D224-V723) was used as the immunogen for the TSR1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TSR1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the TSR1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for TSR1

Target: TSR1 (TSR1, 20S rRNA Accumulation, Homolog (TSR1))

Alternative Name: TSR1

Background: TSR1 antibody detects Pre-rRNA-processing protein TSR1 homolog, a ribosome biogenesis factor required for the maturation of the small ribosomal subunit (40S). TSR1 is an essential nucleolar protein that participates in the late stages of pre-18S rRNA processing, ensuring proper ribosome assembly and translation efficiency. The TSR1 antibody is used in studies of ribosome formation, RNA metabolism, and cellular growth control.

TSR1 is encoded by the TSR1 gene located on human chromosome 17q25.3. The protein is approximately 88 kilodaltons and conserved across eukaryotes, underscoring its critical role in ribosome biogenesis. TSR1 associates with pre-ribosomal particles in the nucleolus and cytoplasm and functions during the transition of 90S to 40S ribosomal precursors. It acts as a structural scaffold that stabilizes pre-rRNA intermediates and recruits processing enzymes necessary for final 18S rRNA cleavage.

The TSR1 antibody typically detects an 88-90 kilodalton band on western blot and is useful for immunofluorescence studies showing nucleolar and cytoplasmic localization. Knockdown or mutation of TSR1 disrupts ribosome biogenesis, leading to impaired protein synthesis and activation of nucleolar stress pathways such as p53 stabilization. As a core assembly factor, TSR1 interacts with RIO kinases, NOB1, and other late-acting ribosome maturation proteins.

In addition to its canonical role in ribosome formation, TSR1 may contribute to cell cycle progression and proliferation through its control of global translation. Aberrant expression of TSR1 has been observed in several cancers, suggesting a link between elevated ribosome production and tumorigenesis. NSJ Bioreagents provides a validated TSR1 antibody, supporting detailed studies of ribosome assembly, nucleolar dynamics, and translation regulation.

UniProt: Q2NL82

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly