POMT1 antibody (AA 224-747)

Catalog No. ABIN7872396

The Rabbit Polyclonal anti-POMT1 antibody is suitable to detect POMT1 in samples from Human and Rat. It has been validated for WB, ELISA and FACS.

Quick Overview for POMT1 antibody (AA 224-747) (ABIN7872396)

Target: POMT1 (Protein-O-Mannosyltransferase 1 (POMT1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POMT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-POMT1 Antibody

Binding Specificity: AA 224-747

Purpose: POMT1 Antibody / Protein O-mannosyl-transferase 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human POMT1 recombinant protein (Position: H224-H747) was used as the immunogen for the POMT1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the POMT1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the POMT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for POMT1

Target: POMT1 (Protein-O-Mannosyltransferase 1 (POMT1))

Alternative Name: POMT1

Background: POMT1 antibody detects Protein O-mannosyl-transferase 1, an enzyme required for the initial step of O-mannosyl glycan biosynthesis on glycoproteins, particularly alpha-dystroglycan. The UniProt recommended name is Protein O-mannosyl-transferase 1 (POMT1). This enzyme catalyzes the transfer of mannose from dolichol phosphate-mannose to serine or threonine residues on target proteins, a process essential for proper protein folding, stability, and function.

Functionally, POMT1 antibody identifies a 747-amino-acid endoplasmic reticulum (ER) membrane protein that forms a functional heterodimer with POMT2. This complex initiates the O-mannosyl glycosylation pathway, which is critical for dystroglycan processing and muscle integrity. POMT1 ensures the attachment of the first mannose residue, forming the foundation for subsequent glycan extensions that link the extracellular matrix to the cytoskeleton via alpha-dystroglycan.

The POMT1 gene is located on chromosome 9q34.13 and is expressed in brain, skeletal muscle, and heart. It plays a vital role in muscle development, neuronal migration, and synaptic organization by supporting glycosylation-dependent cell adhesion and signaling.

Pathologically, mutations in POMT1 cause congenital muscular dystrophy-dystroglycanopathy type A1 (Walker-Warburg syndrome), type B1, and type C1. These disorders are characterized by muscular dystrophy, brain malformations, and ocular defects due to defective O-mannosylation of dystroglycan. Research using POMT1 antibody supports studies in glycosylation pathways, muscular dystrophy, and neuronal development.

POMT1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect ER glycosyltransferases. NSJ Bioreagents provides POMT1 antibody reagents optimized for studies in protein modification, muscle biology, and glycoprotein processing.

Structurally, Protein O-mannosyl-transferase 1 contains multiple transmembrane domains and a luminal catalytic domain harboring the conserved DXD motif required for glycosyltransferase activity. It assembles with POMT2 to form an enzymatically active complex that determines substrate recognition and catalysis. This antibody aids in investigating POMT1i 1/2s role in glycoprotein biosynthesis, muscle development, and congenital disease mechanisms.

UniProt: Q9Y6A1