PAN3 antibody (AA 227-887)

Catalog No. ABIN7872414

The Rabbit Polyclonal anti-PAN3 antibody is suitable to detect PAN3 in samples from Human and Mouse. It has been validated for WB, ELISA, FACS, IHC, IF and ICC.

Quick Overview for PAN3 antibody (AA 227-887) (ABIN7872414)

Target: PAN3 (PAN3 Poly(A) Specific Ribonuclease Subunit Homolog (PAN3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAN3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PAN3 Antibody

Binding Specificity: AA 227-887

Purpose: PAN3 Antibody / PAN deadenylation complex subunit 3

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human PAN3 recombinant protein (Position: Q227-L887) was used as the immunogen for the PAN3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PAN3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PAN3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PAN3

Target: PAN3 (PAN3 Poly(A) Specific Ribonuclease Subunit Homolog (PAN3))

Alternative Name: PAN3

Background: PAN3 antibody detects PAB-dependent poly(A)-specific ribonuclease subunit 3, a component of the PAN deadenylation complex responsible for the removal of poly(A) tails from mRNA molecules. PAN3 forms a heterodimer with PAN2 to mediate mRNA turnover, regulating transcript stability and translation. The PAN3 antibody is widely used in studies of RNA metabolism, post-transcriptional control, and gene expression regulation.

PAN3 is encoded by the PAN3 gene located on human chromosome 13q12.13. The protein is approximately 89 kilodaltons and characterized by coiled-coil, zinc finger, and RNA-binding motifs that support its role in mRNA degradation. Within the PAN2-PAN3 complex, PAN3 acts as a scaffold protein that recruits PAN2 to poly(A)-bound PABP (poly(A)-binding protein) and stimulates deadenylase activity. This process represents the first step in mRNA decay, preceding decapping and exonucleolytic degradation.

The PAN3 antibody detects an ~96 kilodalton protein by western blot and shows cytoplasmic punctate localization consistent with processing bodies (P-bodies) and stress granules. Functional assays reveal that PAN3, together with PAN2, regulates transcript half-life during stress, differentiation, and cell cycle transitions. Depletion of PAN3 stabilizes mRNAs and alters global translation, underscoring its central role in RNA homeostasis.

Beyond mRNA decay, PAN3 contributes to early embryonic development and stress responses by coordinating the balance between translation and degradation. It interacts with the CCR4-NOT complex and RNA helicases, integrating deadenylation with other post-transcriptional mechanisms. NSJ Bioreagents provides a validated PAN3 antibody optimized for western blot, immunofluorescence, and RNA-binding protein studies, supporting analysis of mRNA turnover and translational regulation in eukaryotic cells.

UniProt: Q58A45