ICOSLG antibody (AA 23-149)

Catalog No. ABIN7872485

This Mouse Monoclonal antibody specifically detects ICOSLG in IHC (p). It exhibits reactivity toward Human.

Quick Overview for ICOSLG antibody (AA 23-149) (ABIN7872485)

Target: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICOSLG antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: ICOSL-3111

Product Details anti-ICOSLG Antibody

Binding Specificity: AA 23-149

Purpose: ICOSLG Antibody / ICOS Ligand / B7RP-1

Purification: Protein G affinity chromatography

Immunogen: A recombinant fragment of human ICOS Ligand / B7RP-1 protein within amino acids 23-149 was used as the immunogen for the ICOSLG antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the ICOSLG antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the ICOSLG antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for ICOSLG

Target: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Alternative Name: ICOSLG

Background: This MAb recognizes a protein of 36 kDa, identified as ICOSLG, which is also known as B7-related protein-1 (B7RP-1).T-cell co-stimulatory molecule, inducible co-stimulator (ICOS)/B7-related protein-1 (B7RP-1, B7-H2, GL50, ICOS-L) is a ligand for the ICOS receptor that initiates T and B cell proliferation and cytokine secretion. ICOS-L interactions play an essential role in T cell-dependent B cell activation in peripheral lymphoid organs such as spleen and lymph nodes. ICOS-L protein is present in myeloid leukocytes, and by northern blot there are 2.4, 3.0, and 7.0 kb transcripts in brain, heart, kidney, and liver, with lower expression in colon and thymus, and a 1.1 kb transcript in leukocytes. Tumor necrosis factor alpha (TNFi±), granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) enhance its expression. LPS-induced up-regulation of ICOS-L is dependent on the MyD88-dependent signaling pathway.

UniProt: O75144

Pathways: Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation