THEM6 antibody (AA 23-208)

Catalog No. ABIN7872503

This Rabbit Polyclonal antibody specifically detects THEM6 in WB, ELISA, ICC, FACS, IF and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for THEM6 antibody (AA 23-208) (ABIN7872503)

Target: THEM6 (Mesenchymal Stem Cell Protein DSCD75 (THEM6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This THEM6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-THEM6 Antibody

Binding Specificity: AA 23-208

Purpose: THEM6 Antibody / Thioesterase superfamily member 6

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human THEM6 recombinant protein (Position: Y23-Q208) was used as the immunogen for the THEM6 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the THEM6 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the THEM6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for THEM6

Target: THEM6 (Mesenchymal Stem Cell Protein DSCD75 (THEM6))

Alternative Name: THEM6

Background: THEM6 antibody detects Acyl-CoA thioesterase THEM6, a lipid-metabolizing enzyme that hydrolyzes fatty acyl-CoA esters to free fatty acids and CoA, regulating lipid signaling and energy homeostasis. The UniProt recommended name is Acyl-CoA thioesterase THEM6 (THEM6). This enzyme belongs to the thioesterase superfamily, which plays essential roles in lipid metabolism, peroxisomal function, and cellular stress response.

Functionally, THEM6 antibody identifies a 247-amino-acid protein localized primarily in the endoplasmic reticulum (ER). THEM6 catalyzes the hydrolysis of long-chain acyl-CoA molecules, controlling intracellular levels of activated fatty acids and maintaining the balance between lipid synthesis and degradation. By regulating acyl-CoA and free fatty acid pools, THEM6 influences energy metabolism, membrane composition, and lipid signaling cascades.

The THEM6 gene is located on chromosome 1p36.33 and encodes an enzyme expressed in metabolically active tissues, including liver, adipose tissue, and skeletal muscle. THEM6 contains a hotdog-fold thioesterase domain characteristic of enzymes that process acyl-CoA intermediates. Its activity is critical for preventing the accumulation of toxic acyl-CoA derivatives that can impair mitochondrial function and oxidative metabolism. THEM6 also participates in lipid remodeling pathways that maintain ER membrane homeostasis.

In cellular metabolism, THEM6 acts as a regulator of lipid droplet dynamics and fatty acid oxidation. It modulates the availability of CoA for beta-oxidation and phospholipid biosynthesis, indirectly influencing mitochondrial energy production. Dysregulation of THEM6 expression has been linked to metabolic disorders, including fatty liver disease and obesity. Emerging evidence suggests a role for THEM6 in cancer cell metabolism, where enhanced lipid turnover supports rapid proliferation and membrane biogenesis.

THEM6 antibody is widely used in lipid metabolism, enzymology, and metabolic disease research. It is suitable for western blotting, immunofluorescence, and subcellular localization studies to detect THEM6 expression in cellular and tissue samples. This antibody supports studies of acyl-CoA metabolism, lipid homeostasis, and ER stress responses. In metabolic and cancer research, THEM6 detection provides insights into how cells reprogram lipid utilization under stress or nutrient limitation.

Structurally, THEM6 contains a conserved thioesterase catalytic triad composed of serine, histidine, and aspartate residues, mediating acyl-CoA hydrolysis. Its ER localization is maintained through a C-terminal retention signal, and its expression may be regulated by nutrient and hormonal cues. NSJ Bioreagents provides THEM6 antibody reagents validated for use in lipid signaling, metabolic regulation, and enzymatic function research.

UniProt: Q8WUY1