MCMBP antibody (AA 234-534)

Catalog No. ABIN7872605

This Rabbit Polyclonal antibody specifically detects MCMBP in WB, ELISA and IP. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for MCMBP antibody (AA 234-534) (ABIN7872605)

Target: MCMBP (Minichromosome Maintenance Complex Binding Protein (MCMBP))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCMBP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-MCMBP Antibody

Binding Specificity: AA 234-534

Purpose: MCMBP Antibody / MCM-binding protein

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human MCMBP recombinant protein (Position: E234-E534) was used as the immunogen for the MCMBP antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MCMBP antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MCMBP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MCMBP

Target: MCMBP (Minichromosome Maintenance Complex Binding Protein (MCMBP))

Alternative Name: MCMBP

Background: MCMBP antibody detects Minichromosome maintenance complex-binding protein, a nuclear protein that regulates DNA replication and cell cycle progression. MCMBP interacts with the MCM (minichromosome maintenance) helicase complex, ensuring its proper assembly and nuclear localization. It functions as a chaperone stabilizing MCM subunits before DNA replication initiation. The MCMBP antibody is valuable in studies of DNA replication licensing, genome stability, and cell proliferation control.

MCMBP is encoded by the MCMBP gene located on human chromosome 10q24.2. The protein is approximately 70 kilodaltons in size and associates with MCM subunits MCM2-MCM7. These interactions facilitate the transport of MCM complexes into the nucleus and their incorporation into pre-replication complexes at replication origins. MCMBP assists in maintaining replication fork integrity and prevents unscheduled replication events that could lead to genomic instability. It also contributes to the disassembly of replication machinery during mitotic exit.

The MCMBP antibody identifies the 70 kilodalton protein by western blot and reveals nuclear localization during S phase, with partial cytoplasmic presence in early G1. Immunofluorescence shows co-localization with replication foci, supporting its role in DNA synthesis regulation. Functional studies demonstrate that depletion of MCMBP causes replication stress, reduced origin firing, and accumulation of DNA damage markers such as gamma-H2AX. MCMBP interacts with replication factor C and proliferating cell nuclear antigen, integrating replication initiation with elongation control.

Beyond DNA replication, MCMBP is involved in maintaining chromosomal stability and has been linked to mitotic checkpoint regulation. Overexpression has been reported in certain cancers, including hepatocellular and colorectal carcinomas, where it may promote cell cycle progression. Conversely, its loss sensitizes cells to replication inhibitors, suggesting potential as a therapeutic target. NSJ Bioreagents provides a validated MCMBP antibody optimized for western blot and immunoprecipitation, supporting detailed exploration of replication licensing, genome integrity, and cell cycle dynamics.

UniProt: Q9BTE3

Pathways: Chromatin Binding