LHB antibody (AA 24-140)

Catalog No. ABIN7872666

The Rabbit Polyclonal anti-LHB antibody is suitable to detect LHB in samples from Zebrafish (Danio rerio). It has been validated for IHC (p).

Quick Overview for LHB antibody (AA 24-140) (ABIN7872666)

Target: LHB (Luteinizing Hormone beta Polypeptide (LHB))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LHB antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LHB Antibody

Binding Specificity: AA 24-140

Purpose: Zebrafish Luteinizing hormone beta Antibody / Lhb

Purification: Antigen affinity chromatography

Immunogen: E. coli-derived zebrafish Luteinizing hormone beta recombinant protein (amino acids L24-Y140) was used as the immunogen for the Zebrafish Luteinizing hormone beta antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal dilution of the Zebrafish Luteinizing hormone beta antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Zebrafish Luteinizing hormone beta antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for LHB

Target: LHB (Luteinizing Hormone beta Polypeptide (LHB))

Alternative Name: Luteinizing hormone beta

Target Type: Hormone

Background: Luteinizing hormone (LH) is a glycoprotein hormone critical for the regulation of reproductive function in vertebrates. It consists of a common alpha subunit and a hormone-specific beta subunit, the latter conferring biological specificity. In zebrafish (Danio rerio), the Lhb gene encodes the beta subunit of LH and plays a key role in the hypothalamic-pituitary-gonadal (HPG) axis.

Zebrafish Lhb is produced by gonadotrope cells in the anterior pituitary and is essential for triggering final oocyte maturation and ovulation in females, as well as steroidogenesis and spermatogenesis in males. Its expression is tightly regulated by gonadotropin-releasing hormone (GnRH) and modulated by sex steroids through feedback mechanisms.

The zebrafish model has been instrumental in dissecting the molecular and developmental regulation of lhb expression. Notably, Lhb mRNA becomes detectable during larval stages and increases during sexual maturation. Functional studies using gene knockout and reporter lines have demonstrated that Lhb is indispensable for fertility, and its disruption results in reproductive failure.

Due to its conserved structure and function, zebrafish Lhb serves as a valuable model for studying reproductive endocrinology, pituitary cell differentiation, and hormone signaling pathways in vertebrates.

UniProt: Q6TCF5

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity, C21-Steroid Hormone Metabolic Process