PLD5 antibody (AA 247-517)

Catalog No. ABIN7872808

The Rabbit Polyclonal anti-PLD5 antibody (ABIN7872808) specifically detects PLD5 in ELISA, WB, IHC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PLD5 antibody (AA 247-517) (ABIN7872808)

Target: PLD5 (Phospholipase D Family, Member 5 (PLD5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLD5 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-PLD5 Antibody

Binding Specificity: AA 247-517

Purpose: PLD5 Antibody / Phospholipase D family member 5

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human PLD5 recombinant protein (Position: K247-K517) was used as the immunogen for the PLD5 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PLD5 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PLD5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PLD5

Target: PLD5 (Phospholipase D Family, Member 5 (PLD5))

Alternative Name: PLD5

Background: PLD5 antibody detects Phospholipase D family member 5, encoded by the PLD5 gene on chromosome 1q43. Phospholipase D enzymes catalyze hydrolysis of phosphatidylcholine to produce phosphatidic acid, a lipid signaling molecule that regulates cellular pathways including vesicle trafficking, cytoskeletal dynamics, and signal transduction. PLD5, however, is considered catalytically inactive due to substitutions in critical catalytic residues. Despite lacking enzymatic activity, PLD5 has been implicated in neuronal development, apoptosis, and cellular signaling, suggesting non-canonical functions that distinguish it from PLD1 and PLD2.

Expression of PLD5 is enriched in the brain, particularly in regions such as the hippocampus and cerebellum, where it may influence synaptic plasticity and neuronal survival. Experimental studies suggest that PLD5 regulates apoptosis pathways, possibly through scaffolding functions or interactions with other lipid signaling proteins. Its expression is developmentally regulated, peaking during neuronal differentiation. These observations make PLD5 an intriguing candidate for research into brain development and neurodegeneration.

Although PLD5 is enzymatically inactive, it remains structurally related to other phospholipase D family proteins. It contains conserved phox homology (PX) and pleckstrin homology (PH) domains, which are typically involved in membrane targeting and protein interactions. These domains suggest that PLD5 may function as a regulatory scaffold that influences lipid signaling networks. Comparative studies with PLD1 and PLD2 demonstrate how inactive family members such as PLD5 contribute to broader signaling diversity. PLD5 antibody is therefore an essential reagent for distinguishing PLD5 expression patterns from active family members and for clarifying its regulatory roles.

Research applications of PLD5 antibody include western blotting, immunohistochemistry, and immunofluorescence, especially in brain tissues. It has been used to track expression during neuronal differentiation and in models of neurodegeneration. By comparing PLD5 expression to catalytically active phospholipases, researchers gain insight into how non-enzymatic family members influence signaling. NSJ Bioreagents provides PLD5 antibody for these applications, enabling the study of this understudied but significant protein.

UniProt: Q8N7P1