SPON1 antibody (AA 247-611)

Catalog No. ABIN7872810

The Rabbit Polyclonal anti-SPON1 antibody is suitable to detect SPON1 in samples from Human. It has been validated for IHC and ELISA.

Quick Overview for SPON1 antibody (AA 247-611) (ABIN7872810)

Target: SPON1 (Spondin 1 (SPON1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPON1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Product Details anti-SPON1 Antibody

Binding Specificity: AA 247-611

Purpose: SPON1 Antibody / Spondin 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SPON1 recombinant protein (Position: E247-H611) was used as the immunogen for the SPON1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SPON1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SPON1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SPON1

Target: SPON1 (Spondin 1 (SPON1))

Alternative Name: SPON1

Background: SPON1 antibody detects Spondin-1, an extracellular matrix protein that promotes cell adhesion, axon guidance, and tissue organization. Spondin-1 is a member of the F-spondin family of secreted glycoproteins that regulate neuronal outgrowth and vascular patterning. The SPON1 antibody is used in neuroscience, developmental biology, and vascular research to study ECM signaling and neural development.

SPON1 is encoded by the SPON1 gene located on human chromosome 11p15.2. The protein is approximately 91 kilodaltons and consists of a signal peptide, reelin domain, thrombospondin type I repeats, and a C-terminal region that mediates cell surface binding. Spondin-1 is secreted into the extracellular matrix where it interacts with integrins and heparan sulfate proteoglycans to promote neurite extension and cell attachment.

A SPON1 antibody can detect a 90-95 kilodalton band by western blot and reveals extracellular and pericellular staining in immunohistochemistry. During embryonic development, Spondin-1 guides commissural axons across the midline and contributes to axonal fasciculation. In adult tissues, it participates in vascular remodeling and ECM integrity. Spondin-1 is also implicated in Alzheimer's disease pathology, where altered expression affects amyloid precursor protein processing.

Beyond neural function, Spondin-1 contributes to chondrogenesis, osteogenesis, and tumor angiogenesis. Its expression is upregulated during wound repair and inflammation, suggesting a role in tissue regeneration. NSJ Bioreagents provides a validated SPON1 antibody, supporting investigations into neuronal growth and extracellular signaling.

UniProt: Q9HCB6