HDDC3 antibody (AA 25-179)

Catalog No. ABIN7872846

The Rabbit Polyclonal anti-HDDC3 antibody is suitable to detect HDDC3 in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for HDDC3 antibody (AA 25-179) (ABIN7872846)

Target: HDDC3 (HD Domain Containing 3 (HDDC3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDDC3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HDDC3 Antibody

Binding Specificity: AA 25-179

Purpose: HDDC3 Antibody / HD domain-containing protein 3

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human HDDC3 recombinant protein (Position: K25-I179) was used as the immunogen for the HDDC3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the HDDC3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the HDDC3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for HDDC3

Target: HDDC3 (HD Domain Containing 3 (HDDC3))

Alternative Name: HDDC3

Background: HDDC3 antibody detects HD domain-containing protein 3, a mitochondrial enzyme involved in NAD metabolism and stress-induced apoptosis regulation. The UniProt recommended name is HD domain-containing protein 3 (HDDC3). Also known as mitochondrial stress response protein MESH1, this enzyme exhibits NADPH phosphatase activity and modulates cellular redox state during stress adaptation.

Functionally, HDDC3 antibody identifies a 246-amino-acid protein localized to the mitochondrial matrix and cytoplasm. HDDC3 hydrolyzes NADPH to NADP+, regulating redox balance and reactive oxygen species production. It serves as a mammalian homolog of bacterial stringent response hydrolases, linking metabolic control to cell survival and autophagy.

The HDDC3 gene is located on chromosome 9q21.12 and is widely expressed, with highest levels in liver, kidney, and brain. Under oxidative or nutrient stress, HDDC3 modulates mitochondrial function, suppresses excessive NADPH accumulation, and contributes to redox homeostasis.

Pathologically, dysregulation of HDDC3 affects metabolic stress responses and apoptosis. Loss of function sensitizes cells to oxidative damage, while overexpression has been associated with tumor progression and altered NADPH signaling. Research using HDDC3 antibody supports studies in metabolism, mitochondrial dynamics, and cell death pathways.

HDDC3 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect NADPH-regulating enzymes. NSJ Bioreagents provides HDDC3 antibody reagents optimized for studies in mitochondrial metabolism, apoptosis, and redox biology.

Structurally, HD domain-containing protein 3 contains a conserved metal-dependent hydrolase domain that coordinates divalent cations for catalysis. This antibody enables analysis of HDDC3's enzymatic role in maintaining redox equilibrium and mitochondrial resilience.

UniProt: Q8N4P3