APOA1 antibody (AA 25-264)

Catalog No. ABIN7872866

This Mouse Monoclonal antibody specifically detects APOA1 in WB and ELISA (Capture). It exhibits reactivity toward Human.

Quick Overview for APOA1 antibody (AA 25-264) (ABIN7872866)

Target: APOA1 (Apolipoprotein A-I (APOA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This APOA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA (Capture)

Clone: 17G5

Product Details anti-APOA1 Antibody

Binding Specificity: AA 25-264

Purpose: APOA1 Antibody / Apolipoprotein A

Purification: Affinity-chromatography

Immunogen: E. coli-derived mouse Apolipoprotein A I recombinant protein (Position: D25-Q264) was used as the immunogen for the APOA1 antibody.

Isotype: IgG1

Application Details

Application Notes: Optimal dilution of the APOA1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the APOA1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for APOA1

Target: APOA1 (Apolipoprotein A-I (APOA1))

Alternative Name: APOA1

Background: APOA1 antibody detects Apolipoprotein A-I, the major protein component of high-density lipoprotein (HDL) that mediates cholesterol transport and lipid metabolism. APOA1 facilitates reverse cholesterol transport by binding cellular cholesterol and phospholipids, delivering them to the liver for excretion. The APOA1 antibody is widely used in cardiovascular, metabolic, and lipid biology research to study HDL function, atherosclerosis prevention, and lipid-protein interactions.

APOA1 is encoded by the APOA1 gene located on human chromosome 11q23.3. The protein is approximately 243 amino acids long and synthesized mainly in the liver and small intestine. APOA1 circulates in plasma as part of HDL particles, where it activates lecithin-cholesterol acyltransferase (LCAT), an enzyme that esterifies free cholesterol, promoting HDL maturation. It plays a central role in maintaining lipid balance and preventing lipid accumulation in arterial walls.

The APOA1 antibody detects a 28 kilodalton band by western blot and exhibits strong cytoplasmic and extracellular staining in hepatic and intestinal tissues. APOA1 interacts with ATP-binding cassette transporters ABCA1 and ABCG1 to facilitate cholesterol efflux from peripheral cells. It also exhibits antioxidant and anti-inflammatory functions by neutralizing oxidized lipids and modulating cytokine signaling, contributing to cardiovascular protection.

Mutations or decreased expression of APOA1 lead to hypoalphalipoproteinemia and increased risk of coronary artery disease. Conversely, elevated APOA1 levels correlate with improved lipid clearance and reduced atherosclerotic plaque formation. In addition to lipid transport, APOA1 influences glucose metabolism, immune modulation, and amyloid aggregation prevention.

As a multifunctional lipid carrier and protective factor in cardiovascular health, APOA1 serves as both a biomarker and therapeutic target for metabolic disorders. NSJ Bioreagents provides a validated APOA1 antibody optimized for western blot, supporting research into HDL biology, cholesterol regulation, and cardiovascular disease prevention.

UniProt: P02647

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism