SHARPIN antibody (AA 25-325)

Catalog No. ABIN7872872

This Rabbit Polyclonal antibody specifically detects SHARPIN in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for SHARPIN antibody (AA 25-325) (ABIN7872872)

Target: SHARPIN (SHANK-Associated RH Domain Interacting Protein (SHARPIN))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHARPIN antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-SHARPIN Antibody

Binding Specificity: AA 25-325

Purpose: SHARPIN Antibody / SHANK-associated RH domain-interacting protein

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SHARPIN recombinant protein (Position: H25-R325) was used as the immunogen for the SHARPIN antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SHARPIN antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SHARPIN antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SHARPIN

Target: SHARPIN (SHANK-Associated RH Domain Interacting Protein (SHARPIN))

Alternative Name: SHARPIN

Background: SHARPIN antibody detects SHANK-associated RH domain-interacting protein, a multifunctional adaptor protein involved in inflammation, apoptosis, and cytoskeletal signaling. The UniProt recommended name is SHANK-associated RH domain-interacting protein (SHARPIN). This cytoplasmic protein is a key component of the linear ubiquitin chain assembly complex (LUBAC), which modulates NF-kappaB activation and immune response signaling.

Functionally, SHARPIN antibody recognizes a 387-amino-acid protein that acts as a scaffolding subunit linking the ubiquitin ligase HOIP (RNF31) and HOIL-1L (RBCK1) to form the active LUBAC complex. This complex catalyzes linear (M1-linked) polyubiquitination of signaling proteins, stabilizing TNF receptor-associated complexes and promoting NF-kappaB activation. Through this pathway, SHARPIN regulates innate and adaptive immune responses, cell survival, and inflammatory gene transcription. It also negatively regulates apoptotic signaling by inhibiting caspase activation downstream of TNF receptors.

The SHARPIN gene is located on chromosome 8q24.3 and encodes a widely expressed cytoplasmic protein enriched in immune cells, skin, and neural tissues. Beyond immune regulation, SHARPIN interacts with integrins and actin-associated proteins to control cell adhesion and motility. It binds to the SHANK family of postsynaptic scaffolds, linking cytoskeletal dynamics to synaptic stability and plasticity. SHARPIN thus functions as both an immune modulator and structural adaptor in distinct cellular contexts.

Deficiency or mutation of SHARPIN disrupts LUBAC assembly, leading to impaired NF-kappaB signaling and increased apoptosis. Mouse models lacking SHARPIN exhibit severe chronic inflammation and skin lesions, known as the chronic proliferative dermatitis (cpdm) phenotype. In humans, SHARPIN dysregulation has been implicated in inflammatory diseases, cancer, and neurodegeneration. Elevated SHARPIN expression correlates with tumor progression in prostate and breast cancers, where it enhances PI3K/AKT signaling and resistance to apoptosis.

SHARPIN antibody is widely used for research into inflammatory signaling, ubiquitination, and immune regulation. Applications include immunoblotting, immunohistochemistry, and co-immunoprecipitation to analyze LUBAC complex formation and function. Its detection provides insight into TNF receptor signaling, cell death control, and integrin-mediated adhesion. In neurobiology, SHARPIN interacts with SHANK scaffolds and PSD-95 at excitatory synapses, influencing postsynaptic organization and neuronal communication.

Structurally, SHARPIN contains an N-terminal PH domain-like motif, a central ubiquitin-like (UBL) domain, and a C-terminal Npl4 zinc finger (NZF) domain responsible for binding linear ubiquitin chains. Post-translational regulation includes phosphorylation and ubiquitination, controlling its participation in signaling networks. NSJ Bioreagents provides SHARPIN antibody reagents validated for use in inflammation, ubiquitin signaling, and neurobiology research.

UniProt: Q9H0F6