GPC2 antibody (AA 25-498)

Catalog No. ABIN7872883

The Rabbit Polyclonal anti-GPC2 antibody (ABIN7872883) specifically detects GPC2 in WB, ELISA and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for GPC2 antibody (AA 25-498) (ABIN7872883)

Target: GPC2 (Glypican 2 (GPC2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPC2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-GPC2 Antibody

Binding Specificity: AA 25-498

Purpose: GPC2 Antibody / Glypican 2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human Glypican 2/GPC2 recombinant protein (Position: E25-D498) was used as the immunogen for the GPC2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the GPC2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the GPC2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for GPC2

Target: GPC2 (Glypican 2 (GPC2))

Alternative Name: GPC2

Background: The GPC2 antibody targets Glypican-2, a cell surface heparan sulfate proteoglycan encoded by the GPC2 gene. Glypican-2 is a member of the glypican family that anchors to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage. It modulates signaling pathways such as Wnt, Hedgehog, and fibroblast growth factor (FGF), thereby influencing cell proliferation, differentiation, and neural development. The GPC2 antibody provides researchers with a high-specificity reagent to study membrane-associated signaling regulation and neurodevelopmental processes.

Glypican-2 is predominantly expressed in the developing nervous system, where it regulates neurite outgrowth and synaptic organization. By binding heparan sulfate chains to morphogens and growth factors, GPC2 fine-tunes signal transduction gradients essential for brain patterning. The GPC2 antibody supports detection of this proteoglycan in neuronal cultures and brain tissue, allowing exploration of its role in axonal guidance and synaptic plasticity. GPC2 also interacts with cell adhesion molecules to influence neuronal connectivity during development.

Recent research has identified Glypican-2 as a cell surface oncoprotein in pediatric neuroblastoma. Overexpression of GPC2 enhances cell proliferation and tumor growth through sustained activation of oncogenic signaling pathways. The GPC2 antibody supports investigation of this function, enabling measurement of expression levels in tumor samples and evaluation of therapeutic targeting potential. Antibody-drug conjugates and immunotherapies directed against GPC2 are currently under study for selective tumor eradication.

Beyond neuroblastoma, Glypican-2 contributes to neuronal regeneration and neural stem cell regulation. Its heparan sulfate chains bind multiple signaling ligands, positioning GPC2 as a key modulator of microenvironmental signaling. The GPC2 antibody helps map these ligandi 1/2receptor interactions and track expression changes during neuronal differentiation. Reduced expression of GPC2 has been linked to impaired synaptic signaling, suggesting its importance in maintaining neural circuit stability.

The GPC2 antibody is validated for western blotting, flow cytometry, and immunofluorescence, showing clear cell surface localization consistent with GPI-anchored proteins. NSJ Bioreagents provides this antibody as a validated reagent for reproducible detection in developmental, neurological, and oncology research. By supporting detailed study of Glypican-2 structure and function, the GPC2 antibody advances understanding of membrane-bound proteoglycans in cell signaling and neuroblastoma biology.

UniProt: Q8N158

Pathways: Glycosaminoglycan Metabolic Process